TY - JOUR
T1 - Glyphosate Resistance in Eleusine indica
T2 - Involvement of CYP71AK44 in Addition to EPSPS Gene Overexpression
AU - He, Sifen
AU - Tian, Junhui
AU - Ouyang, Yulan
AU - Liao, Yuxi
AU - Yu, Qin
AU - Bai, Lianyang
AU - Pan, Lang
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024/10/30
Y1 - 2024/10/30
N2 - Intensive application of glyphosate has resulted in resistance evolution in many weed populations, including Eleusine indica. This study characterized glyphosate resistance and investigated the underlying mechanisms in a glyphosate-resistant population (R-JX) of E. indica from China. The R-JX population was 8.5 times resistant to glyphosate relative to the glyphosate-susceptible population (SA). Point mutations were not observed in the target gene 5-enolypyruvyl-shikimate-3-phosphate synthase gene (EPSPS). However, the expression level and copy number of EPSPS were 8.8 times and 15.2 times, respectively, greater in R-JX than that in the SA population. Pre-application of the P450 inhibitor lowered the resistance level to glyphosate from 8.5 times to 3.6 times in the R-JX population. RNA-Seq and RT-qPCR revealed that the CYP71AK44 gene was consistently upregulated in R-JX and five other glyphosate-resistant populations. Rice calli and seedlings overexpressing CYP71AK44 showed glyphosate resistance. In conclusion, overexpression of the target EPSPS plus CYP71AK44 collectively contributes to glyphosate resistance in these E. indica populations.
AB - Intensive application of glyphosate has resulted in resistance evolution in many weed populations, including Eleusine indica. This study characterized glyphosate resistance and investigated the underlying mechanisms in a glyphosate-resistant population (R-JX) of E. indica from China. The R-JX population was 8.5 times resistant to glyphosate relative to the glyphosate-susceptible population (SA). Point mutations were not observed in the target gene 5-enolypyruvyl-shikimate-3-phosphate synthase gene (EPSPS). However, the expression level and copy number of EPSPS were 8.8 times and 15.2 times, respectively, greater in R-JX than that in the SA population. Pre-application of the P450 inhibitor lowered the resistance level to glyphosate from 8.5 times to 3.6 times in the R-JX population. RNA-Seq and RT-qPCR revealed that the CYP71AK44 gene was consistently upregulated in R-JX and five other glyphosate-resistant populations. Rice calli and seedlings overexpressing CYP71AK44 showed glyphosate resistance. In conclusion, overexpression of the target EPSPS plus CYP71AK44 collectively contributes to glyphosate resistance in these E. indica populations.
KW - CYP71AK44
KW - EPSPS overexpression
KW - non-target-site resistance (NTSR)
UR - http://www.scopus.com/inward/record.url?scp=85205969615&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.4c07765
DO - 10.1021/acs.jafc.4c07765
M3 - Article
C2 - 39377301
AN - SCOPUS:85205969615
SN - 0021-8561
VL - 72
SP - 23758
EP - 23765
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 43
ER -