Glyphosate resistance in Ambrosia trifida: Part 2. Rapid response physiology and non-target-site resistance

Marcelo L. Moretti; Christopher R. Van Horn; Renae Robertson; Kabelo Segobye; Stephen C. Weller; Bryan G. Young; William G. Johnson; R. Douglas Sammons; Dafu Wang; Xia Ge; Andre d' Avignon; Todd A. Gaines; Philip Westra; Amanda C. Green; Taylor Jeffery; Mackenzie A. Lesperance; Francois J. Tardif; Peter H. Sikkema; J. Christopher Hall; Michael D. McLean; Mark B. Lawton; Burkhard Schulz

doi:10.1002/ps.4569

Glyphosate resistance in Ambrosia trifida: Part 2. Rapid response physiology and non-target-site resistance

Marcelo L. Moretti, Christopher R. Van Horn, Renae Robertson, Kabelo Segobye, Stephen C. Weller, Bryan G. Young, William G. Johnson, R. Douglas Sammons, Dafu Wang, Xia Ge, Andre d' Avignon, Todd A. Gaines, Philip Westra, Amanda C. Green, Taylor Jeffery, Mackenzie A. Lesperance, Francois J. Tardif, Peter H. Sikkema, J. Christopher Hall, Michael D. McLeanMark B. Lawton, Burkhard Schulz

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Abstract

BACKGROUND The glyphosate-resistant rapid response (GR RR) resistance mechanism in Ambrosia trifida is not due to target-site resistance (TSR) mechanisms. This study explores the physiology of the rapid response and the possibility of reduced translocation and vacuolar sequestration as non-target-site resistance (NTSR) mechanisms. RESULTS GR RR leaf discs accumulated hydrogen peroxide within minutes of glyphosate exposure, but only in mature leaf tissue. The rapid response required energy either as light or exogenous sucrose. The combination of phenylalanine and tyrosine inhibited the rapid response in a dose-dependent manner. Reduced glyphosate translocation was observed in GR RR, but only when associated with tissue death caused by the rapid response. Nuclear magnetic resonance studies indicated that glyphosate enters the cytoplasm and reaches chloroplasts, and it is not moved into the vacuole of GR RR, GR non-rapid response or glyphosate-susceptible A. trifida. CONCLUSION The GR RR mechanism of resistance is not associated with vacuole sequestration of glyphosate, and the observed reduced translocation is likely a consequence of rapid tissue death. Rapid cell death was inhibited by exogenous application of aromatic amino acids phenylalanine and tyrosine. The mechanism by which these amino acids inhibit rapid cell death in the GR RR phenotype remains unknown, and it could involve glyphosate phytotoxicity or other agents generating reactive oxygen species. Implications of these findings are discussed. The GR RR mechanism is distinct from the currently described glyphosate TSR or NTSR mechanisms in other species.

Original language	English
Pages (from-to)	1079-1088
Number of pages	10
Journal	Pest Management Science
Volume	74
Issue number	5
DOIs	https://doi.org/10.1002/ps.4569
Publication status	Published - May 2018
Externally published	Yes
Event	Symposium on Glyphosate held at the 252nd Annual Fall Meeting of the American-Chemical-Society (ACS) - Philadelphia, Panama Duration: 1 Jan 2016 → …

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Moretti, M. L., Van Horn, C. R., Robertson, R., Segobye, K., Weller, S. C., Young, B. G., Johnson, W. G., Sammons, R. D., Wang, D., Ge, X., d' Avignon, A., Gaines, T. A., Westra, P., Green, A. C., Jeffery, T., Lesperance, M. A., Tardif, F. J., Sikkema, P. H., Hall, J. C., ... Schulz, B. (2018). Glyphosate resistance in Ambrosia trifida: Part 2. Rapid response physiology and non-target-site resistance. Pest Management Science, 74(5), 1079-1088. https://doi.org/10.1002/ps.4569

@article{fac2189983ef415080b006997a72e859,

title = "Glyphosate resistance in Ambrosia trifida: Part 2. Rapid response physiology and non-target-site resistance",

abstract = "BACKGROUND The glyphosate-resistant rapid response (GR RR) resistance mechanism in Ambrosia trifida is not due to target-site resistance (TSR) mechanisms. This study explores the physiology of the rapid response and the possibility of reduced translocation and vacuolar sequestration as non-target-site resistance (NTSR) mechanisms. RESULTS GR RR leaf discs accumulated hydrogen peroxide within minutes of glyphosate exposure, but only in mature leaf tissue. The rapid response required energy either as light or exogenous sucrose. The combination of phenylalanine and tyrosine inhibited the rapid response in a dose-dependent manner. Reduced glyphosate translocation was observed in GR RR, but only when associated with tissue death caused by the rapid response. Nuclear magnetic resonance studies indicated that glyphosate enters the cytoplasm and reaches chloroplasts, and it is not moved into the vacuole of GR RR, GR non-rapid response or glyphosate-susceptible A. trifida. CONCLUSION The GR RR mechanism of resistance is not associated with vacuole sequestration of glyphosate, and the observed reduced translocation is likely a consequence of rapid tissue death. Rapid cell death was inhibited by exogenous application of aromatic amino acids phenylalanine and tyrosine. The mechanism by which these amino acids inhibit rapid cell death in the GR RR phenotype remains unknown, and it could involve glyphosate phytotoxicity or other agents generating reactive oxygen species. Implications of these findings are discussed. The GR RR mechanism is distinct from the currently described glyphosate TSR or NTSR mechanisms in other species. ",

keywords = "Ambrosia trifida, Giant ragweed, Nuclear magnetic resonance, Rapid response, Sequestration, Translocation",

author = "Moretti, {Marcelo L.} and {Van Horn}, {Christopher R.} and Renae Robertson and Kabelo Segobye and Weller, {Stephen C.} and Young, {Bryan G.} and Johnson, {William G.} and Sammons, {R. Douglas} and Dafu Wang and Xia Ge and {d' Avignon}, Andre and Gaines, {Todd A.} and Philip Westra and Green, {Amanda C.} and Taylor Jeffery and Lesperance, {Mackenzie A.} and Tardif, {Francois J.} and Sikkema, {Peter H.} and Hall, {J. Christopher} and McLean, {Michael D.} and Lawton, {Mark B.} and Burkhard Schulz",

year = "2018",

month = may,

doi = "10.1002/ps.4569",

language = "English",

volume = "74",

pages = "1079--1088",

journal = "Pest Management Science",

issn = "1526-498X",

publisher = "John Wiley & Sons",

number = "5",

note = "Symposium on Glyphosate held at the 252nd Annual Fall Meeting of the American-Chemical-Society (ACS) ; Conference date: 01-01-2016",

}

Moretti, ML, Van Horn, CR, Robertson, R, Segobye, K, Weller, SC, Young, BG, Johnson, WG, Sammons, RD, Wang, D, Ge, X, d' Avignon, A, Gaines, TA, Westra, P, Green, AC, Jeffery, T, Lesperance, MA, Tardif, FJ, Sikkema, PH, Hall, JC, McLean, MD, Lawton, MB & Schulz, B 2018, 'Glyphosate resistance in Ambrosia trifida: Part 2. Rapid response physiology and non-target-site resistance', Pest Management Science, vol. 74, no. 5, pp. 1079-1088. https://doi.org/10.1002/ps.4569

TY - JOUR

T1 - Glyphosate resistance in Ambrosia trifida

T2 - Symposium on Glyphosate held at the 252nd Annual Fall Meeting of the American-Chemical-Society (ACS)

AU - Moretti, Marcelo L.

AU - Van Horn, Christopher R.

AU - Robertson, Renae

AU - Segobye, Kabelo

AU - Weller, Stephen C.

AU - Young, Bryan G.

AU - Johnson, William G.

AU - Sammons, R. Douglas

AU - Wang, Dafu

AU - Ge, Xia

AU - d' Avignon, Andre

AU - Gaines, Todd A.

AU - Westra, Philip

AU - Green, Amanda C.

AU - Jeffery, Taylor

AU - Lesperance, Mackenzie A.

AU - Tardif, Francois J.

AU - Sikkema, Peter H.

AU - Hall, J. Christopher

AU - McLean, Michael D.

AU - Lawton, Mark B.

AU - Schulz, Burkhard

PY - 2018/5

Y1 - 2018/5

N2 - BACKGROUND The glyphosate-resistant rapid response (GR RR) resistance mechanism in Ambrosia trifida is not due to target-site resistance (TSR) mechanisms. This study explores the physiology of the rapid response and the possibility of reduced translocation and vacuolar sequestration as non-target-site resistance (NTSR) mechanisms. RESULTS GR RR leaf discs accumulated hydrogen peroxide within minutes of glyphosate exposure, but only in mature leaf tissue. The rapid response required energy either as light or exogenous sucrose. The combination of phenylalanine and tyrosine inhibited the rapid response in a dose-dependent manner. Reduced glyphosate translocation was observed in GR RR, but only when associated with tissue death caused by the rapid response. Nuclear magnetic resonance studies indicated that glyphosate enters the cytoplasm and reaches chloroplasts, and it is not moved into the vacuole of GR RR, GR non-rapid response or glyphosate-susceptible A. trifida. CONCLUSION The GR RR mechanism of resistance is not associated with vacuole sequestration of glyphosate, and the observed reduced translocation is likely a consequence of rapid tissue death. Rapid cell death was inhibited by exogenous application of aromatic amino acids phenylalanine and tyrosine. The mechanism by which these amino acids inhibit rapid cell death in the GR RR phenotype remains unknown, and it could involve glyphosate phytotoxicity or other agents generating reactive oxygen species. Implications of these findings are discussed. The GR RR mechanism is distinct from the currently described glyphosate TSR or NTSR mechanisms in other species.

AB - BACKGROUND The glyphosate-resistant rapid response (GR RR) resistance mechanism in Ambrosia trifida is not due to target-site resistance (TSR) mechanisms. This study explores the physiology of the rapid response and the possibility of reduced translocation and vacuolar sequestration as non-target-site resistance (NTSR) mechanisms. RESULTS GR RR leaf discs accumulated hydrogen peroxide within minutes of glyphosate exposure, but only in mature leaf tissue. The rapid response required energy either as light or exogenous sucrose. The combination of phenylalanine and tyrosine inhibited the rapid response in a dose-dependent manner. Reduced glyphosate translocation was observed in GR RR, but only when associated with tissue death caused by the rapid response. Nuclear magnetic resonance studies indicated that glyphosate enters the cytoplasm and reaches chloroplasts, and it is not moved into the vacuole of GR RR, GR non-rapid response or glyphosate-susceptible A. trifida. CONCLUSION The GR RR mechanism of resistance is not associated with vacuole sequestration of glyphosate, and the observed reduced translocation is likely a consequence of rapid tissue death. Rapid cell death was inhibited by exogenous application of aromatic amino acids phenylalanine and tyrosine. The mechanism by which these amino acids inhibit rapid cell death in the GR RR phenotype remains unknown, and it could involve glyphosate phytotoxicity or other agents generating reactive oxygen species. Implications of these findings are discussed. The GR RR mechanism is distinct from the currently described glyphosate TSR or NTSR mechanisms in other species.

KW - Ambrosia trifida

KW - Giant ragweed

KW - Nuclear magnetic resonance

KW - Rapid response

KW - Sequestration

KW - Translocation

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=uwapure5-25&SrcAuth=WosAPI&KeyUT=WOS:000428524600009&DestLinkType=FullRecord&DestApp=WOS

U2 - 10.1002/ps.4569

DO - 10.1002/ps.4569

M3 - Article

C2 - 28276187

SN - 1526-498X

VL - 74

SP - 1079

EP - 1088

JO - Pest Management Science

JF - Pest Management Science

IS - 5

Y2 - 1 January 2016

ER -

Glyphosate resistance in Ambrosia trifida: Part 2. Rapid response physiology and non-target-site resistance

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