TY - JOUR
T1 - Glycogen synthetase in the sea mussel Mytilus edulis L.-I. Purification, interconversion and kinetic properties of the I and D forms
AU - Cook, P. A.
AU - Gabbott, P. A.
N1 - Funding Information:
Acknowledoements--P. A. Cook thanks Professor D. J. Crisp, F.R.S. for providing accommodation at Menai Bridge, and the NERC and C.S.I.R. (South Africa) for providing financial support. Dr. B. L. Bayne and Dr D. R. Livingstone kindly reviewed the manuscript.
PY - 1978
Y1 - 1978
N2 - 1. 1. The two forms, I (active, independent of G6P) and D (inactive, dependent on G6P), of glycogen synthetase have been identified in Mytilus edulis. 2. 2. Incubation of a crude extract of the mantle enzyme at 30°C resulted in conversion of the D to I form due to endogenous phosphatase activity. 3. 3. The mantle enzyme was purified by affinity chromatography on Con A-Sepharose. The Km values for UDPG were 0.32 and 2.8 mM for the D and I forms of the enzyme, respectively. The Ka for G6P, of the D form was 0.76 mM. 4. 4. Inorganic phosphate (2-10 mM) strongly inhibited the D form of glycogen synthetase. The inhibition was not reversed by increased concentrations of G6P (10 mM); in contrast, 5 mM G6P reversed the inhibition of the I form by inorganic phosphate.
AB - 1. 1. The two forms, I (active, independent of G6P) and D (inactive, dependent on G6P), of glycogen synthetase have been identified in Mytilus edulis. 2. 2. Incubation of a crude extract of the mantle enzyme at 30°C resulted in conversion of the D to I form due to endogenous phosphatase activity. 3. 3. The mantle enzyme was purified by affinity chromatography on Con A-Sepharose. The Km values for UDPG were 0.32 and 2.8 mM for the D and I forms of the enzyme, respectively. The Ka for G6P, of the D form was 0.76 mM. 4. 4. Inorganic phosphate (2-10 mM) strongly inhibited the D form of glycogen synthetase. The inhibition was not reversed by increased concentrations of G6P (10 mM); in contrast, 5 mM G6P reversed the inhibition of the I form by inorganic phosphate.
UR - http://www.scopus.com/inward/record.url?scp=0344066807&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(78)90071-8
DO - 10.1016/0305-0491(78)90071-8
M3 - Article
AN - SCOPUS:0344066807
SN - 1096-4959
VL - 60
SP - 419
EP - 421
JO - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
IS - 4
ER -