TY - JOUR
T1 - Gibberellin Production by Newly Isolated Strain Leifsonia soli SE134 and Its Potential to Promote Plant Growth
AU - Kang, Sang-Mo
AU - Khan, Abdul Latif
AU - You, Young-Hyun
AU - Kim, Jong-Guk
AU - Kamran, Muhammad
AU - Lee, In-Jung
PY - 2014/1/28
Y1 - 2014/1/28
N2 - Very few plant growth-promoting rhizobacteria (PGPR) are known to produce gibberellins (GAs). The current study aimed to isolate a phytohormone-producing PGP rhizobacterium from soil and assess its potential to enhance plant growth. The newly isolated bacterium was identified as Leifsonia soli sp. SE134 on the basis of partial 16S ribosomal RNA gene sequence. Application of L. soli culture filtrate significantly increased the biomass, hypocotyl, and root lengths of cucumber seeds as compared with non-inoculated sole medium and distilled water treated controls. Furthermore, the PGPR culture was applied to the GA-deficient mutant rice cultivar Waito-C. Treatment with L. soli SE134 significantly increased the growth of Waito-C rice seedlings as compared with controls. Upon chromatographic analysis of L. soli culture, we isolated, detected and quantified different GAs; namely, GA(1) (0.61 +/- 0.15), GA(4) (1.58 +/- 0.26), GA(7) (0.54 +/- 0.18), GA(8)(0.98 +/- 0.15), GA(9), (0.45 +/- 0.17), GA(12)(0.64 +/- 0.21), GA(19) (0.18 +/- 0.09), GA(20) (0.78 +/- 0.15), GA(24) (0.38 +/- 0.09), GA(34) (0.35 +/- 0.10), and GA(53) (0.17 +/- 0.05). Plant growth promotion in cucumber, tomato, and young radish plants further evidenced the potential of this strain as a PGP bacterium. The results suggest that GA secretion by L. soli SE134 might prove advantageous for its ameliorative role in crop growth. These findings can be extended for improving the productivity of different crops under diverse environmental conditions.
AB - Very few plant growth-promoting rhizobacteria (PGPR) are known to produce gibberellins (GAs). The current study aimed to isolate a phytohormone-producing PGP rhizobacterium from soil and assess its potential to enhance plant growth. The newly isolated bacterium was identified as Leifsonia soli sp. SE134 on the basis of partial 16S ribosomal RNA gene sequence. Application of L. soli culture filtrate significantly increased the biomass, hypocotyl, and root lengths of cucumber seeds as compared with non-inoculated sole medium and distilled water treated controls. Furthermore, the PGPR culture was applied to the GA-deficient mutant rice cultivar Waito-C. Treatment with L. soli SE134 significantly increased the growth of Waito-C rice seedlings as compared with controls. Upon chromatographic analysis of L. soli culture, we isolated, detected and quantified different GAs; namely, GA(1) (0.61 +/- 0.15), GA(4) (1.58 +/- 0.26), GA(7) (0.54 +/- 0.18), GA(8)(0.98 +/- 0.15), GA(9), (0.45 +/- 0.17), GA(12)(0.64 +/- 0.21), GA(19) (0.18 +/- 0.09), GA(20) (0.78 +/- 0.15), GA(24) (0.38 +/- 0.09), GA(34) (0.35 +/- 0.10), and GA(53) (0.17 +/- 0.05). Plant growth promotion in cucumber, tomato, and young radish plants further evidenced the potential of this strain as a PGP bacterium. The results suggest that GA secretion by L. soli SE134 might prove advantageous for its ameliorative role in crop growth. These findings can be extended for improving the productivity of different crops under diverse environmental conditions.
KW - Gibberellins
KW - plant growth promotion
KW - PGPR
KW - Leifsonia soli
KW - SP NOV.
KW - AZOSPIRILLUM-BRASILENSE
KW - RHIZOBACTERIA
KW - CULTURE
KW - RICE
KW - BIOSYNTHESIS
KW - RHIZOSPHERE
KW - METABOLIZE
KW - SEEDLINGS
KW - BACTERIA
UR - http://www.scopus.com/inward/record.url?scp=84893122607&partnerID=8YFLogxK
U2 - 10.4014/jmb.1304.04015
DO - 10.4014/jmb.1304.04015
M3 - Article
SN - 1017-7825
VL - 24
SP - 106
EP - 112
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
IS - 1
ER -