Genomic structure and bacteriophages of Moraxella catarrhalis

Amir Mohamed Hassan

Genomic structure and bacteriophages of Moraxella catarrhalis

Amir Mohamed Hassan

Research output: Thesis › Doctoral Thesis

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Abstract

Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes was
demonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Chang, Barbara, Supervisor Kahler, Charlene, Supervisor
Award date	1 Mar 2017
Publication status	Unpublished - 2017

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THESIS - DOCTOR OF PHILOSOPHY - MOHAMED HASSAN, Amir Ariff - 2017
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title = "Genomic structure and bacteriophages of Moraxella catarrhalis",

abstract = "Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes wasdemonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.",

keywords = "Pan-genome, bacteriophage, Whole genome sequencing, Multi locus sequence typing, Sero-sensitivity, Virulence factor, Genomic analysis, Moraxella catarrhalis",

author = "{Mohamed Hassan}, Amir",

year = "2017",

language = "English",

school = "The University of Western Australia",

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TY - THES

T1 - Genomic structure and bacteriophages of Moraxella catarrhalis

AU - Mohamed Hassan, Amir

PY - 2017

Y1 - 2017

N2 - Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes wasdemonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.

AB - Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes wasdemonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.

KW - Pan-genome

KW - bacteriophage

KW - Whole genome sequencing

KW - Multi locus sequence typing

KW - Sero-sensitivity

KW - Virulence factor

KW - Genomic analysis

KW - Moraxella catarrhalis

M3 - Doctoral Thesis

ER -

Genomic structure and bacteriophages of Moraxella catarrhalis

Abstract

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