Genomic structure and bacteriophages of Moraxella catarrhalis

Amir Mohamed Hassan

    Research output: ThesisDoctoral Thesis

    Abstract

    Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan­ genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes was
    demonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.

    LanguageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • The University of Western Australia
    Award date1 Mar 2017
    StateUnpublished - 2017

    Fingerprint

    Moraxella catarrhalis
    bacteriophages
    genomics
    genome
    genes
    Siphoviridae
    nutrient uptake
    microscopy
    virulence
    viruses
    pathogens

    Cite this

    @phdthesis{c7e4c6f8b57645b78487ec67d22a6ae5,
    title = "Genomic structure and bacteriophages of Moraxella catarrhalis",
    abstract = "Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan­ genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes wasdemonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.",
    keywords = "Pan-genome, bacteriophage, Whole genome sequencing, Multi locus sequence typing, Sero-sensitivity, Virulence factor, Genomic analysis, Moraxella catarrhalis",
    author = "{Mohamed Hassan}, Amir",
    year = "2017",
    language = "English",
    school = "The University of Western Australia",

    }

    Mohamed Hassan, A 2017, 'Genomic structure and bacteriophages of Moraxella catarrhalis', Doctor of Philosophy, The University of Western Australia.

    Genomic structure and bacteriophages of Moraxella catarrhalis. / Mohamed Hassan, Amir.

    2017.

    Research output: ThesisDoctoral Thesis

    TY - THES

    T1 - Genomic structure and bacteriophages of Moraxella catarrhalis

    AU - Mohamed Hassan,Amir

    PY - 2017

    Y1 - 2017

    N2 - Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan­ genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes wasdemonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.

    AB - Moraxella catarrhalis is an important bacterial respiratory pathogen. This thesis sought to add to currently limited knowledge of M. catarrhalis genomic structure. High-throughput sequenc ing was used to obtain 86 M. catarrhalis whole genomes. Pan­ genome analysis revealed cladal differences in nutrient uptake genes and CRISPRs. The diversity of virulence genes wasdemonstrated in relation to disease presentation, host age, strain source and cladal structure. Evolutionary modelling revealed unexpected pressures affecting these genes. Thirty-two novel Siphoviridae-related prophages, viral genomes within the bacterial chromososomes, were described, which harboured hyperconserved genes. Epifluorescence microscopy provided the first demonstration of active viruses in M. catarrhalis.

    KW - Pan-genome

    KW - bacteriophage

    KW - Whole genome sequencing

    KW - Multi locus sequence typing

    KW - Sero-sensitivity

    KW - Virulence factor

    KW - Genomic analysis

    KW - Moraxella catarrhalis

    M3 - Doctoral Thesis

    ER -