Genomic sequence and host range studies reveal considerable variation within the species Arracacha virus B

Roger A.C. Jones, Adrian Fox, Neil Boonham, Ian P. Adams

Research output: Contribution to journalArticle

Abstract

Arracacha virus B type (AVB-T) and oca (AVB-O) strains from arracacha (Arracacia xanthorrhiza) and oca (Oxalis tuberosa) samples collected in 1975 and two additional isolates obtained from arracacha (AVB-PX) and potato (AVB-6A) in Peru in 1976 and 1978, respectively, were studied. In its host responses and serological properties, AVB-PX most resembled AVB-T, whereas AVB-6A most resembled AVB-O. Complete genomic sequences of the RNA-1 and RNA-2 of each isolate were obtained following high-throughput sequencing of RNA extracts from isolates preserved for 38 (AVB-PX) or 32 (the other 3 isolates) years, and compared with a genomic sequence of AVB-O obtained previously (PV-0082). RNA-2 was unexpectedly divergent compared to RNA-1, with the nucleotide (nt) sequence identity of different AVB isolates varying by up to 76% (RNA-2) and 89% (RNA-1). The coat protein amino acid sequences were the most divergent, with AVB-O and AVB-6A having only 68% identity to AVB-T and AVB-PX. Since the RNA2 sequence differences between the two isolate groupings also coincided with host range, symptom, and serological differences, AVB demonstrates considerable intraspecific divergence.

Original languageEnglish
Pages (from-to)2849-2852
Number of pages4
JournalArchives of Virology
Volume164
Issue number11
DOIs
Publication statusPublished - 1 Nov 2019

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Cercopithecine Herpesvirus 1
Host Specificity
RNA
High-Throughput Nucleotide Sequencing
Peru
Capsid Proteins
Solanum tuberosum
Amino Acid Sequence

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Jones, Roger A.C. ; Fox, Adrian ; Boonham, Neil ; Adams, Ian P. / Genomic sequence and host range studies reveal considerable variation within the species Arracacha virus B. In: Archives of Virology. 2019 ; Vol. 164, No. 11. pp. 2849-2852.
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abstract = "Arracacha virus B type (AVB-T) and oca (AVB-O) strains from arracacha (Arracacia xanthorrhiza) and oca (Oxalis tuberosa) samples collected in 1975 and two additional isolates obtained from arracacha (AVB-PX) and potato (AVB-6A) in Peru in 1976 and 1978, respectively, were studied. In its host responses and serological properties, AVB-PX most resembled AVB-T, whereas AVB-6A most resembled AVB-O. Complete genomic sequences of the RNA-1 and RNA-2 of each isolate were obtained following high-throughput sequencing of RNA extracts from isolates preserved for 38 (AVB-PX) or 32 (the other 3 isolates) years, and compared with a genomic sequence of AVB-O obtained previously (PV-0082). RNA-2 was unexpectedly divergent compared to RNA-1, with the nucleotide (nt) sequence identity of different AVB isolates varying by up to 76{\%} (RNA-2) and 89{\%} (RNA-1). The coat protein amino acid sequences were the most divergent, with AVB-O and AVB-6A having only 68{\%} identity to AVB-T and AVB-PX. Since the RNA2 sequence differences between the two isolate groupings also coincided with host range, symptom, and serological differences, AVB demonstrates considerable intraspecific divergence.",
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Genomic sequence and host range studies reveal considerable variation within the species Arracacha virus B. / Jones, Roger A.C.; Fox, Adrian; Boonham, Neil; Adams, Ian P.

In: Archives of Virology, Vol. 164, No. 11, 01.11.2019, p. 2849-2852.

Research output: Contribution to journalArticle

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