Genomic analysis of oral Campylobacter concisus strains identified a potential bacterial molecular marker associated with active Crohn's disease

Fang Liu, Rena Ma, Chin Yen Alfred Tay, Sophie Octavia, Ruiting Lan, Heung Kit Leslie Chung, Stephen M. Riordan, Michael C. Grimm, Rupert W. Leong, Mark M. Tanaka, Susan Connor, Li Zhang

Research output: Contribution to journalArticle

  • 2 Citations

Abstract

Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease (IBD) including Crohn's disease (CD) and ulcerative colitis (UC). C. concisus consists of two genomospecies (GS) and diverse strains. This study aimed to identify molecular markers to differentiate commensal and IBD-associated C. concisus strains. The genomes of 63 oral C. concisus strains isolated from patients with IBD and healthy controls were examined, of which 38 genomes were sequenced in this study. We identified a novel secreted enterotoxin B homologue, Csep1. The csep1 gene was found in 56% of GS2 C. concisus strains, presented in the plasmid pICON or the chromosome. A six-nucleotide insertion at the position 654-659 bp in csep1 (csep1-6bpi) was found. The presence of csep1-6bpi in oral C. concisus strains isolated from patients with active CD (47%, 7/15) was significantly higher than that in strains from healthy controls (0/29, P = 0.0002), and the prevalence of csep1-6bpi positive C. concisus strains was significantly higher in patients with active CD (67%, 4/6) as compared to healthy controls (0/23, P = 0.0006). Proteomics analysis detected the Csep1 protein. A csep1 gene hot spot in the chromosome of different C. concisus strains was found. The pICON plasmid was only found in GS2 strains isolated from the two relapsed CD patients with small bowel complications. This study reports a C. concisus molecular marker (csep1-6bpi) that is associated with active CD.

LanguageEnglish
Article number64
JournalEmerging Microbes and Infections
Volume7
Issue number1
DOIs
StatePublished - 1 Dec 2018

Fingerprint

Campylobacter
Crohn Disease
Inflammatory Bowel Diseases
Plasmids
Chromosomes, Human, 6-12 and X
Genome
Enterotoxins
Ulcerative Colitis
Proteomics
Genes
Nucleotides
Chromosomes
Bacteria
Proteins

Cite this

Liu, Fang ; Ma, Rena ; Tay, Chin Yen Alfred ; Octavia, Sophie ; Lan, Ruiting ; Chung, Heung Kit Leslie ; Riordan, Stephen M. ; Grimm, Michael C. ; Leong, Rupert W. ; Tanaka, Mark M. ; Connor, Susan ; Zhang, Li. / Genomic analysis of oral Campylobacter concisus strains identified a potential bacterial molecular marker associated with active Crohn's disease. In: Emerging Microbes and Infections. 2018 ; Vol. 7, No. 1.
@article{55ad98a810b64d709ca9eafd50ba2bf6,
title = "Genomic analysis of oral Campylobacter concisus strains identified a potential bacterial molecular marker associated with active Crohn's disease",
abstract = "Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease (IBD) including Crohn's disease (CD) and ulcerative colitis (UC). C. concisus consists of two genomospecies (GS) and diverse strains. This study aimed to identify molecular markers to differentiate commensal and IBD-associated C. concisus strains. The genomes of 63 oral C. concisus strains isolated from patients with IBD and healthy controls were examined, of which 38 genomes were sequenced in this study. We identified a novel secreted enterotoxin B homologue, Csep1. The csep1 gene was found in 56{\%} of GS2 C. concisus strains, presented in the plasmid pICON or the chromosome. A six-nucleotide insertion at the position 654-659 bp in csep1 (csep1-6bpi) was found. The presence of csep1-6bpi in oral C. concisus strains isolated from patients with active CD (47{\%}, 7/15) was significantly higher than that in strains from healthy controls (0/29, P = 0.0002), and the prevalence of csep1-6bpi positive C. concisus strains was significantly higher in patients with active CD (67{\%}, 4/6) as compared to healthy controls (0/23, P = 0.0006). Proteomics analysis detected the Csep1 protein. A csep1 gene hot spot in the chromosome of different C. concisus strains was found. The pICON plasmid was only found in GS2 strains isolated from the two relapsed CD patients with small bowel complications. This study reports a C. concisus molecular marker (csep1-6bpi) that is associated with active CD.",
author = "Fang Liu and Rena Ma and Tay, {Chin Yen Alfred} and Sophie Octavia and Ruiting Lan and Chung, {Heung Kit Leslie} and Riordan, {Stephen M.} and Grimm, {Michael C.} and Leong, {Rupert W.} and Tanaka, {Mark M.} and Susan Connor and Li Zhang",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41426-018-0065-6",
language = "English",
volume = "7",
journal = "Emerging Microbes and Infections",
issn = "2222-1751",
publisher = "Nature Publishing Group",
number = "1",

}

Genomic analysis of oral Campylobacter concisus strains identified a potential bacterial molecular marker associated with active Crohn's disease. / Liu, Fang; Ma, Rena; Tay, Chin Yen Alfred; Octavia, Sophie; Lan, Ruiting; Chung, Heung Kit Leslie; Riordan, Stephen M.; Grimm, Michael C.; Leong, Rupert W.; Tanaka, Mark M.; Connor, Susan; Zhang, Li.

In: Emerging Microbes and Infections, Vol. 7, No. 1, 64, 01.12.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genomic analysis of oral Campylobacter concisus strains identified a potential bacterial molecular marker associated with active Crohn's disease

AU - Liu,Fang

AU - Ma,Rena

AU - Tay,Chin Yen Alfred

AU - Octavia,Sophie

AU - Lan,Ruiting

AU - Chung,Heung Kit Leslie

AU - Riordan,Stephen M.

AU - Grimm,Michael C.

AU - Leong,Rupert W.

AU - Tanaka,Mark M.

AU - Connor,Susan

AU - Zhang,Li

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease (IBD) including Crohn's disease (CD) and ulcerative colitis (UC). C. concisus consists of two genomospecies (GS) and diverse strains. This study aimed to identify molecular markers to differentiate commensal and IBD-associated C. concisus strains. The genomes of 63 oral C. concisus strains isolated from patients with IBD and healthy controls were examined, of which 38 genomes were sequenced in this study. We identified a novel secreted enterotoxin B homologue, Csep1. The csep1 gene was found in 56% of GS2 C. concisus strains, presented in the plasmid pICON or the chromosome. A six-nucleotide insertion at the position 654-659 bp in csep1 (csep1-6bpi) was found. The presence of csep1-6bpi in oral C. concisus strains isolated from patients with active CD (47%, 7/15) was significantly higher than that in strains from healthy controls (0/29, P = 0.0002), and the prevalence of csep1-6bpi positive C. concisus strains was significantly higher in patients with active CD (67%, 4/6) as compared to healthy controls (0/23, P = 0.0006). Proteomics analysis detected the Csep1 protein. A csep1 gene hot spot in the chromosome of different C. concisus strains was found. The pICON plasmid was only found in GS2 strains isolated from the two relapsed CD patients with small bowel complications. This study reports a C. concisus molecular marker (csep1-6bpi) that is associated with active CD.

AB - Campylobacter concisus is an oral bacterium that is associated with inflammatory bowel disease (IBD) including Crohn's disease (CD) and ulcerative colitis (UC). C. concisus consists of two genomospecies (GS) and diverse strains. This study aimed to identify molecular markers to differentiate commensal and IBD-associated C. concisus strains. The genomes of 63 oral C. concisus strains isolated from patients with IBD and healthy controls were examined, of which 38 genomes were sequenced in this study. We identified a novel secreted enterotoxin B homologue, Csep1. The csep1 gene was found in 56% of GS2 C. concisus strains, presented in the plasmid pICON or the chromosome. A six-nucleotide insertion at the position 654-659 bp in csep1 (csep1-6bpi) was found. The presence of csep1-6bpi in oral C. concisus strains isolated from patients with active CD (47%, 7/15) was significantly higher than that in strains from healthy controls (0/29, P = 0.0002), and the prevalence of csep1-6bpi positive C. concisus strains was significantly higher in patients with active CD (67%, 4/6) as compared to healthy controls (0/23, P = 0.0006). Proteomics analysis detected the Csep1 protein. A csep1 gene hot spot in the chromosome of different C. concisus strains was found. The pICON plasmid was only found in GS2 strains isolated from the two relapsed CD patients with small bowel complications. This study reports a C. concisus molecular marker (csep1-6bpi) that is associated with active CD.

UR - http://www.scopus.com/inward/record.url?scp=85045308796&partnerID=8YFLogxK

U2 - 10.1038/s41426-018-0065-6

DO - 10.1038/s41426-018-0065-6

M3 - Article

VL - 7

JO - Emerging Microbes and Infections

T2 - Emerging Microbes and Infections

JF - Emerging Microbes and Infections

SN - 2222-1751

IS - 1

M1 - 64

ER -