Projects per year
Disease resistance in Brassica juncea has been shown against several diseases including blackleg, white rust, hypocotyl rot, turnip mosaic virus, leaf blight, black rot, and Alternaria blight. The identification of disease resistance genes is important to develop elite cultivars, which can survive exposure to these diseases. The largest family of resistance genes are nucleotide-binding site leucine-rich repeat genes (NLRs) which are classified into two major subfamilies: toll/interleukin-1 receptor-NLR (TNL) and coiled-coil-NLR (CNL) proteins. Here we present the first study of the genomic organization and evolutionary history of the NLR gene family in B. juncea, with comparative analysis to the diploid progenitors B. rapa and B. nigra. A total of 289 NLR genes were identified in B. juncea, with a ratio of 0.61:1 of TNL to non-TNL genes. The distribution of NLR genes was random and uneven, with chromosome A04 containing no NLR genes. Domain structure analysis revealed that 24% of NLR genes are typical resistance genes containing all three domains (TIR/CC, NBS, LRR), whereas the remaining genes are partially deleted or truncated. A total of 45% NLR genes were found to be physically clustered in the B. juncea genome. The NLR genes were analysed with OrthoFinder which showed that most physical clusters (63%) in B. juncea came from the same orthogroup. This study provides a valuable resource for the identification and characterization of candidate NLR genes for Brassica crop improvement.
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- 3 Finished
1/01/16 → 31/12/21
Defining the Brassica Pan-genome and Establishing Methods for Gene Conversion Based Crop Improvement
1/01/14 → 31/12/16
1/01/13 → 31/07/18