TY - JOUR
T1 - Genome-wide identification and comparative analysis of NBS-LRR resistance genes in Brassica napus
AU - Alamery, Salman
AU - Tirnaz, Soodeh
AU - Bayer, Philipp
AU - Tollenaere, Reece
AU - Chaloub, Boulos
AU - Edwards, David
AU - Batley, Jacqueline
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Plant disease-resistance genes play a critical role in providing resistance against pathogens. The largest family of resistance genes are the nucleotide-binding site (NBS) and leucine-rich repeat (LRR) genes. They are classified into two major subfamilies, toll/interleukin-1 receptor (TIR)-NBS-LRR (TNL) and coiled-coil (CC)-NBS-LRR (CNL) proteins. We have identified and characterised 641 NBS-LRR genes in Brassica napus, 249 in B. rapa and 443 in B. oleracea. A ratio of 1:2 of CNL:TNL genes was found in the three species. Domain structure analysis revealed that 57% of the NBS-LRR genes are typical resistance genes and contain all three domains (TIR/CC, NBS, LRR), whereas the remaining genes are partially deleted or truncated. Of the NBS-LRR genes, 59% were found to be physically clustered, and individual genes involved in clusters were more polymorphic than those not clustered. Of the NBS-LRR genes in B. napus, 50% were identified as duplicates, reflecting a high level of genomic duplication and rearrangement. Comparative analysis between B. napus and its progenitor species indicated that >60% of NBS-LRR genes are conserved in B. napus. This study provides a valuable resource for the identification and characterisation of candidate NBS-LRR genes.
AB - Plant disease-resistance genes play a critical role in providing resistance against pathogens. The largest family of resistance genes are the nucleotide-binding site (NBS) and leucine-rich repeat (LRR) genes. They are classified into two major subfamilies, toll/interleukin-1 receptor (TIR)-NBS-LRR (TNL) and coiled-coil (CC)-NBS-LRR (CNL) proteins. We have identified and characterised 641 NBS-LRR genes in Brassica napus, 249 in B. rapa and 443 in B. oleracea. A ratio of 1:2 of CNL:TNL genes was found in the three species. Domain structure analysis revealed that 57% of the NBS-LRR genes are typical resistance genes and contain all three domains (TIR/CC, NBS, LRR), whereas the remaining genes are partially deleted or truncated. Of the NBS-LRR genes, 59% were found to be physically clustered, and individual genes involved in clusters were more polymorphic than those not clustered. Of the NBS-LRR genes in B. napus, 50% were identified as duplicates, reflecting a high level of genomic duplication and rearrangement. Comparative analysis between B. napus and its progenitor species indicated that >60% of NBS-LRR genes are conserved in B. napus. This study provides a valuable resource for the identification and characterisation of candidate NBS-LRR genes.
KW - comparative genomics
KW - disease resistance
KW - gene cluster
KW - gene duplication.
UR - http://www.scopus.com/inward/record.url?scp=85040326475&partnerID=8YFLogxK
U2 - 10.1071/CP17214
DO - 10.1071/CP17214
M3 - Conference article
AN - SCOPUS:85040326475
SN - 1836-0947
VL - 69
SP - 79
EP - 93
JO - Crop and Pasture Science
JF - Crop and Pasture Science
IS - S1
T2 - International Brassica Conference
Y2 - 1 January 2016 through 1 January 2016
ER -