Genome-wide identification and comparative analysis of NBS-LRR resistance genes in Brassica napus

Salman Alamery, Soodeh Tirnaz, Philipp Bayer, Reece Tollenaere, Boulos Chaloub, David Edwards, Jacqueline Batley

Research output: Contribution to journalConference article

28 Citations (Scopus)
447 Downloads (Pure)

Abstract

Plant disease-resistance genes play a critical role in providing resistance against pathogens. The largest family of resistance genes are the nucleotide-binding site (NBS) and leucine-rich repeat (LRR) genes. They are classified into two major subfamilies, toll/interleukin-1 receptor (TIR)-NBS-LRR (TNL) and coiled-coil (CC)-NBS-LRR (CNL) proteins. We have identified and characterised 641 NBS-LRR genes in Brassica napus, 249 in B. rapa and 443 in B. oleracea. A ratio of 1:2 of CNL:TNL genes was found in the three species. Domain structure analysis revealed that 57% of the NBS-LRR genes are typical resistance genes and contain all three domains (TIR/CC, NBS, LRR), whereas the remaining genes are partially deleted or truncated. Of the NBS-LRR genes, 59% were found to be physically clustered, and individual genes involved in clusters were more polymorphic than those not clustered. Of the NBS-LRR genes in B. napus, 50% were identified as duplicates, reflecting a high level of genomic duplication and rearrangement. Comparative analysis between B. napus and its progenitor species indicated that >60% of NBS-LRR genes are conserved in B. napus. This study provides a valuable resource for the identification and characterisation of candidate NBS-LRR genes.

Original languageEnglish
Pages (from-to)79-93
Number of pages15
JournalCrop and Pasture Science
Volume69
Issue numberS1
DOIs
Publication statusPublished - 1 Jan 2018
EventInternational Brassica Conference : Phenomics to Genomics and Everything in Between - Melbourne, Australia
Duration: 1 Jan 20161 Jan 2016

Fingerprint

Dive into the research topics of 'Genome-wide identification and comparative analysis of NBS-LRR resistance genes in Brassica napus'. Together they form a unique fingerprint.

Cite this