TY - JOUR
T1 - Genome-Wide Association Study for Identification of Marker-Trait Associations Conferring Resistance to Scald from Globally Collected Barley Germplasm
AU - Ijaz, Usman
AU - Zhao, Chenchen
AU - Shahbala, Sergey
AU - Zhou, Meixue
N1 - Publisher Copyright:
© 2024 The American Phytopathological Society.
PY - 2024/7
Y1 - 2024/7
N2 - Scald is one of the major economically important foliar diseases in barley, causing up to 40% yield loss in susceptible varieties. The identification of quantitative trait loci and elite alleles that confer resistance to scald is imperative in reducing the threats to barley production. In this study, genome-wide association studies were conducted using a panel of 697 barley genotypes to identify quantitative trait loci for scald resistance. Field experiments were conducted over three consecutive years. Among different models used for genome-wide association studies analysis, FarmCPU was shown to be the best-suited model. Nineteen significant marker-trait associations related to scald resistance were identified across six different chromosomes. Eleven of these marker-trait associations correspond to previously reported scald resistance genes Rrs1, Rrs4, and Rrs2, respectively. Eight novel marker-trait associations were identified in this study, with the candidate genes encoding a diverse class of proteins, including region leucine-rich repeats, AP2/ERF transcription factor, homeodomain-leucine zipper, and protein kinase family proteins. The combination of identified superior alleles significantly reduces disease severity scores. The results will be valuable for marker-assisted breeding for developing scald-resistant varieties.
AB - Scald is one of the major economically important foliar diseases in barley, causing up to 40% yield loss in susceptible varieties. The identification of quantitative trait loci and elite alleles that confer resistance to scald is imperative in reducing the threats to barley production. In this study, genome-wide association studies were conducted using a panel of 697 barley genotypes to identify quantitative trait loci for scald resistance. Field experiments were conducted over three consecutive years. Among different models used for genome-wide association studies analysis, FarmCPU was shown to be the best-suited model. Nineteen significant marker-trait associations related to scald resistance were identified across six different chromosomes. Eleven of these marker-trait associations correspond to previously reported scald resistance genes Rrs1, Rrs4, and Rrs2, respectively. Eight novel marker-trait associations were identified in this study, with the candidate genes encoding a diverse class of proteins, including region leucine-rich repeats, AP2/ERF transcription factor, homeodomain-leucine zipper, and protein kinase family proteins. The combination of identified superior alleles significantly reduces disease severity scores. The results will be valuable for marker-assisted breeding for developing scald-resistant varieties.
KW - barley
KW - disease resistance
KW - marker-assisted breeding
KW - marker-trait association
KW - scald
KW - Support was provided by the Grains Research and Development Corporation
UR - http://www.scopus.com/inward/record.url?scp=85196257661&partnerID=8YFLogxK
U2 - 10.1094/PHYTO-01-24-0043-R
DO - 10.1094/PHYTO-01-24-0043-R
M3 - Article
C2 - 38451589
AN - SCOPUS:85196257661
SN - 0031-949X
VL - 114
SP - 1637
EP - 1645
JO - Phytopathology
JF - Phytopathology
IS - 7
ER -