Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis

C. Lurin; C. Andres; S. Aubourg; M. Bellaoui; F. Bitton; C. Bruyere; M. Caboche; C. Debast; J. Gualberto; B. Hoffmann; A. Lecharny; M. Le Ret; M-L. Martin-Magniette; H. Mireau; N. Peeters; J-P. Renou; B. Szurek; L. Taconnat; Ian Small

doi:10.1105/tpc.104.022236

Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis

C. Lurin, C. Andres, S. Aubourg, M. Bellaoui, F. Bitton, C. Bruyere, M. Caboche, C. Debast, J. Gualberto, B. Hoffmann, A. Lecharny, M. Le Ret, M-L. Martin-Magniette, H. Mireau, N. Peeters, J-P. Renou, B. Szurek, L. Taconnat, Ian Small

ARC Centre for Plant Energy Biology

Research output: Contribution to journal › Article › peer-review

1124 Citations (Scopus)

Abstract

The complete sequence of the Arabidopsis thaliana genome revealed thousands of previously unsuspected genes, many ofwhich cannot be ascribed even putative functions. One of the largest and most enigmatic gene families discovered in thisway is characterized by tandem arrays of pentatricopeptide repeats (PPRs). We describe a detailed bioinformatic analysis of441 members of the Arabidopsis PPR family plus genomic and genetic data on the expression (microarray data), localization(green fluorescent protein and red fluorescent protein fusions), and general function (insertion mutants and RNA bindingassays) of many family members. The basic picture that arises from these studies is that PPR proteins play constitutive,often essential roles in mitochondria and chloroplasts, probably via binding to organellar transcripts. These results confirm,but massively extend, the very sparse observations previously obtained from detailed characterization of individual mutantsin other organisms.

Original language	English
Pages (from-to)	2089-2103
Journal	The Plant Cell
Volume	16
Issue number	8
DOIs	https://doi.org/10.1105/tpc.104.022236
Publication status	Published - 2004

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Lurin, C., Andres, C., Aubourg, S., Bellaoui, M., Bitton, F., Bruyere, C., Caboche, M., Debast, C., Gualberto, J., Hoffmann, B., Lecharny, A., Le Ret, M., Martin-Magniette, M.-L., Mireau, H., Peeters, N., Renou, J.-P., Szurek, B., Taconnat, L., & Small, I. (2004). Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis. The Plant Cell, 16(8), 2089-2103. https://doi.org/10.1105/tpc.104.022236

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title = "Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis",

abstract = "The complete sequence of the Arabidopsis thaliana genome revealed thousands of previously unsuspected genes, many ofwhich cannot be ascribed even putative functions. One of the largest and most enigmatic gene families discovered in thisway is characterized by tandem arrays of pentatricopeptide repeats (PPRs). We describe a detailed bioinformatic analysis of441 members of the Arabidopsis PPR family plus genomic and genetic data on the expression (microarray data), localization(green fluorescent protein and red fluorescent protein fusions), and general function (insertion mutants and RNA bindingassays) of many family members. The basic picture that arises from these studies is that PPR proteins play constitutive,often essential roles in mitochondria and chloroplasts, probably via binding to organellar transcripts. These results confirm,but massively extend, the very sparse observations previously obtained from detailed characterization of individual mutantsin other organisms.",

author = "C. Lurin and C. Andres and S. Aubourg and M. Bellaoui and F. Bitton and C. Bruyere and M. Caboche and C. Debast and J. Gualberto and B. Hoffmann and A. Lecharny and \{Le Ret\}, M. and M-L. Martin-Magniette and H. Mireau and N. Peeters and J-P. Renou and B. Szurek and L. Taconnat and Ian Small",

year = "2004",

doi = "10.1105/tpc.104.022236",

language = "English",

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pages = "2089--2103",

journal = "The Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

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Lurin, C, Andres, C, Aubourg, S, Bellaoui, M, Bitton, F, Bruyere, C, Caboche, M, Debast, C, Gualberto, J, Hoffmann, B, Lecharny, A, Le Ret, M, Martin-Magniette, M-L, Mireau, H, Peeters, N, Renou, J-P, Szurek, B, Taconnat, L & Small, I 2004, 'Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis', The Plant Cell, vol. 16, no. 8, pp. 2089-2103. https://doi.org/10.1105/tpc.104.022236

TY - JOUR

T1 - Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis

AU - Lurin, C.

AU - Andres, C.

AU - Aubourg, S.

AU - Bellaoui, M.

AU - Bitton, F.

AU - Bruyere, C.

AU - Caboche, M.

AU - Debast, C.

AU - Gualberto, J.

AU - Hoffmann, B.

AU - Lecharny, A.

AU - Le Ret, M.

AU - Martin-Magniette, M-L.

AU - Mireau, H.

AU - Peeters, N.

AU - Renou, J-P.

AU - Szurek, B.

AU - Taconnat, L.

AU - Small, Ian

PY - 2004

Y1 - 2004

N2 - The complete sequence of the Arabidopsis thaliana genome revealed thousands of previously unsuspected genes, many ofwhich cannot be ascribed even putative functions. One of the largest and most enigmatic gene families discovered in thisway is characterized by tandem arrays of pentatricopeptide repeats (PPRs). We describe a detailed bioinformatic analysis of441 members of the Arabidopsis PPR family plus genomic and genetic data on the expression (microarray data), localization(green fluorescent protein and red fluorescent protein fusions), and general function (insertion mutants and RNA bindingassays) of many family members. The basic picture that arises from these studies is that PPR proteins play constitutive,often essential roles in mitochondria and chloroplasts, probably via binding to organellar transcripts. These results confirm,but massively extend, the very sparse observations previously obtained from detailed characterization of individual mutantsin other organisms.

AB - The complete sequence of the Arabidopsis thaliana genome revealed thousands of previously unsuspected genes, many ofwhich cannot be ascribed even putative functions. One of the largest and most enigmatic gene families discovered in thisway is characterized by tandem arrays of pentatricopeptide repeats (PPRs). We describe a detailed bioinformatic analysis of441 members of the Arabidopsis PPR family plus genomic and genetic data on the expression (microarray data), localization(green fluorescent protein and red fluorescent protein fusions), and general function (insertion mutants and RNA bindingassays) of many family members. The basic picture that arises from these studies is that PPR proteins play constitutive,often essential roles in mitochondria and chloroplasts, probably via binding to organellar transcripts. These results confirm,but massively extend, the very sparse observations previously obtained from detailed characterization of individual mutantsin other organisms.

UR - https://www.scopus.com/pages/publications/4043172772

U2 - 10.1105/tpc.104.022236

DO - 10.1105/tpc.104.022236

M3 - Article

SN - 1040-4651

VL - 16

SP - 2089

EP - 2103

JO - The Plant Cell

JF - The Plant Cell

IS - 8

ER -

Genome-Wide Analysis of Arabidopsis Pentatricopeptide Repeat Proteins Reveals Their Essential Role in Organelle Biogenesis

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