TY - JOUR
T1 - Genome-Wide Analysis and Characterization of the Proline-Rich Extensin-like Receptor Kinases (PERKs) Gene Family Reveals Their Role in Different Developmental Stages and Stress Conditions in Wheat (Triticum aestivum L.)
AU - Kesawat, Mahipal Singh
AU - Kherawat, Bhagwat Singh
AU - Singh, Anupama
AU - Dey, Prajjal
AU - Routray, Snehasish
AU - Mohapatra, Chinmayee
AU - Saha, Debanjana
AU - Ram, Chet
AU - Siddique, Kadambot H.M.
AU - Kumar, Ajay
AU - Gupta, Ravi
AU - Chung, Sang Min
AU - Kumar, Manu
PY - 2022/2/1
Y1 - 2022/2/1
N2 - Proline-rich extensin-like receptor kinases (PERKs) are a class of receptor kinases impli-cated in multiple cellular processes in plants. However, there is a lack of information on the PERK gene family in wheat. Therefore, we identified 37 PERK genes in wheat to understand their role in various developmental processes and stress conditions. Phylogenetic analysis of PERK genes from Arabidopsis thaliana, Oryza sativa, Glycine max, and T. aestivum grouped them into eight well-defined classes. Furthermore, synteny analysis revealed 275 orthologous gene pairs in B. distachyon, Ae. tauschii, T. dicoccoides, O. sativa and A. thaliana. Ka/Ks values showed that most TaPERK genes, ex-cept TaPERK1, TaPERK2, TaPERK17, and TaPERK26, underwent strong purifying selection during evolutionary processes. Several cis-acting regulatory elements, essential for plant growth and development and the response to light, phytohormones, and diverse biotic and abiotic stresses, were predicted in the promoter regions of TaPERK genes. In addition, the expression profile of the Ta-PERK gene family revealed differential expression of TaPERK genes in various tissues and developmental stages. Furthermore, TaPERK gene expression was induced by various biotic and abiotic stresses. The RT-qPCR analysis also revealed similar results with slight variation. Therefore, this study’s outcome provides valuable information for elucidating the precise functions of TaPERK in developmental processes and diverse stress conditions in wheat.
AB - Proline-rich extensin-like receptor kinases (PERKs) are a class of receptor kinases impli-cated in multiple cellular processes in plants. However, there is a lack of information on the PERK gene family in wheat. Therefore, we identified 37 PERK genes in wheat to understand their role in various developmental processes and stress conditions. Phylogenetic analysis of PERK genes from Arabidopsis thaliana, Oryza sativa, Glycine max, and T. aestivum grouped them into eight well-defined classes. Furthermore, synteny analysis revealed 275 orthologous gene pairs in B. distachyon, Ae. tauschii, T. dicoccoides, O. sativa and A. thaliana. Ka/Ks values showed that most TaPERK genes, ex-cept TaPERK1, TaPERK2, TaPERK17, and TaPERK26, underwent strong purifying selection during evolutionary processes. Several cis-acting regulatory elements, essential for plant growth and development and the response to light, phytohormones, and diverse biotic and abiotic stresses, were predicted in the promoter regions of TaPERK genes. In addition, the expression profile of the Ta-PERK gene family revealed differential expression of TaPERK genes in various tissues and developmental stages. Furthermore, TaPERK gene expression was induced by various biotic and abiotic stresses. The RT-qPCR analysis also revealed similar results with slight variation. Therefore, this study’s outcome provides valuable information for elucidating the precise functions of TaPERK in developmental processes and diverse stress conditions in wheat.
KW - Drought
KW - Heat stress
KW - Kinase
KW - PERK
KW - Promoter
KW - RT-qPCR
UR - http://www.scopus.com/inward/record.url?scp=85124324539&partnerID=8YFLogxK
U2 - 10.3390/plants11040496
DO - 10.3390/plants11040496
M3 - Article
C2 - 35214830
AN - SCOPUS:85124324539
SN - 2223-7747
VL - 11
JO - Plants
JF - Plants
IS - 4
M1 - 496
ER -