TY - JOUR
T1 - Genetic Diversity of Trichoderma harzianum Isolates in Sunflower Rhizosphere
T2 - The Application of the URP Molecular Marker
AU - Rezaee Danesh, Younes
AU - Pellegrini, Marika
AU - Kariman, Khalil
AU - Boyno, Gökhan
AU - Djebaili, Rihab
AU - Farda, Beatrice
AU - Najafi, Solmaz
N1 - Publisher Copyright:
© 2022 by the authors.
PY - 2022/11
Y1 - 2022/11
N2 - The genetic diversity of 77 Trichoderma harzianum isolates collected from sunflower rhizosphere soils in Urmia, Khoy, and Salmas in West Azerbaijan province, Iran, was evaluated by using the Universal Rice Primer (URP) molecular marker. The DNA band pattern of the isolates was developed using seven primers of this marker. These primers produced 186 gene loci, out of which 182 loci were polymorphic. Accordingly, the genetic diversity of the isolates was calculated, and their kinship relations were determined by cluster analysis using the NTSYS software package. URP-6R had the highest marker index among the studied primers, followed by URP-1F, URP-4R, and URP-25F, implying their higher efficiency in discriminating between the isolates. The results showed that the URP marker could discriminate between isolates using macroscopic morphological characteristics, such as color and colony type, potential of pigment production in the culture medium, and colony growth rate. Furthermore, there was no significant relationship between the geographical distribution of the isolates and the band patterns generated by the primers except for a few cases. The results generally revealed that the URP marker was an efficient tool for determining the genetic diversity of T. harzianum.
AB - The genetic diversity of 77 Trichoderma harzianum isolates collected from sunflower rhizosphere soils in Urmia, Khoy, and Salmas in West Azerbaijan province, Iran, was evaluated by using the Universal Rice Primer (URP) molecular marker. The DNA band pattern of the isolates was developed using seven primers of this marker. These primers produced 186 gene loci, out of which 182 loci were polymorphic. Accordingly, the genetic diversity of the isolates was calculated, and their kinship relations were determined by cluster analysis using the NTSYS software package. URP-6R had the highest marker index among the studied primers, followed by URP-1F, URP-4R, and URP-25F, implying their higher efficiency in discriminating between the isolates. The results showed that the URP marker could discriminate between isolates using macroscopic morphological characteristics, such as color and colony type, potential of pigment production in the culture medium, and colony growth rate. Furthermore, there was no significant relationship between the geographical distribution of the isolates and the band patterns generated by the primers except for a few cases. The results generally revealed that the URP marker was an efficient tool for determining the genetic diversity of T. harzianum.
KW - DNA band pattern
KW - HelianthusL
KW - morphological characteristics
KW - universal rice primer marker
UR - http://www.scopus.com/inward/record.url?scp=85142744952&partnerID=8YFLogxK
U2 - 10.3390/su142215111
DO - 10.3390/su142215111
M3 - Article
AN - SCOPUS:85142744952
SN - 2071-1050
VL - 14
JO - Sustainability (Switzerland)
JF - Sustainability (Switzerland)
IS - 22
M1 - 15111
ER -
