Genetic Association between Swine Leukocyte antigen Class II Haplotypes and Reproduction Traits in Microminipigs

Asako Ando, Noriaki Imaeda, Tatsuya Matsubara, Masaki Takasu, Asuka Miyamoto, Shino Oshima, Naohito Nishii, Yoshie Kametani, Takashi Shiina, Jerzy K. Kulski, Hitoshi Kitagawa

Research output: Contribution to journalArticle

Abstract

The effects of swine leukocyte antigen (SLA) molecules on numerous production and reproduction performance traits have been mainly reported as associations with specific SLA haplotypes that were assigned using serological typing methods. In this study, we intended to clarify the association between SLA class II genes and reproductive traits in a highly inbred population of 187 Microminipigs (MMP), that have eight different types of SLA class II haplotypes. In doing so, we compared the reproductive performances, such as fertility index, gestation period, litter size, and number of stillbirth among SLA class II low resolution haplotypes (Lrs) that were assigned by a polymerase chain reaction-sequence specific primers (PCR-SSP) typing method. Only low resolution haplotypes were used in this study because the eight SLA class II high-resolution haplotypes had been assigned to the 14 parents or the progenitors of the highly inbred MMP herd in a previous publication. The fertility index of dams with Lr-0.13 was significantly lower than that of dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. Dams with Lr-0.23 had significantly smaller litter size at birth than those with Lr-0.17, Lr-0.18, or Lr-0.37. Furthermore, litter size at weaning of dams with Lr-0.23 was also significantly smaller than those dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. The small litter size of dams with Lr-0.23 correlated with the smaller body sizes of these MMPs. These results suggest that SLA class II haplotypes are useful differential genetic markers for further haplotypic and epistatic studies of reproductive traits, selective breeding programs, and improvements in the production and reproduction performances of MMPs.

Original languageEnglish
Article number783
Number of pages13
JournalCells
Volume8
Issue number8
DOIs
Publication statusPublished - Aug 2019

Cite this

Ando, A., Imaeda, N., Matsubara, T., Takasu, M., Miyamoto, A., Oshima, S., ... Kitagawa, H. (2019). Genetic Association between Swine Leukocyte antigen Class II Haplotypes and Reproduction Traits in Microminipigs. Cells, 8(8), [783]. https://doi.org/10.3390/cells8080783
Ando, Asako ; Imaeda, Noriaki ; Matsubara, Tatsuya ; Takasu, Masaki ; Miyamoto, Asuka ; Oshima, Shino ; Nishii, Naohito ; Kametani, Yoshie ; Shiina, Takashi ; Kulski, Jerzy K. ; Kitagawa, Hitoshi. / Genetic Association between Swine Leukocyte antigen Class II Haplotypes and Reproduction Traits in Microminipigs. In: Cells. 2019 ; Vol. 8, No. 8.
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abstract = "The effects of swine leukocyte antigen (SLA) molecules on numerous production and reproduction performance traits have been mainly reported as associations with specific SLA haplotypes that were assigned using serological typing methods. In this study, we intended to clarify the association between SLA class II genes and reproductive traits in a highly inbred population of 187 Microminipigs (MMP), that have eight different types of SLA class II haplotypes. In doing so, we compared the reproductive performances, such as fertility index, gestation period, litter size, and number of stillbirth among SLA class II low resolution haplotypes (Lrs) that were assigned by a polymerase chain reaction-sequence specific primers (PCR-SSP) typing method. Only low resolution haplotypes were used in this study because the eight SLA class II high-resolution haplotypes had been assigned to the 14 parents or the progenitors of the highly inbred MMP herd in a previous publication. The fertility index of dams with Lr-0.13 was significantly lower than that of dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. Dams with Lr-0.23 had significantly smaller litter size at birth than those with Lr-0.17, Lr-0.18, or Lr-0.37. Furthermore, litter size at weaning of dams with Lr-0.23 was also significantly smaller than those dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. The small litter size of dams with Lr-0.23 correlated with the smaller body sizes of these MMPs. These results suggest that SLA class II haplotypes are useful differential genetic markers for further haplotypic and epistatic studies of reproductive traits, selective breeding programs, and improvements in the production and reproduction performances of MMPs.",
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Ando, A, Imaeda, N, Matsubara, T, Takasu, M, Miyamoto, A, Oshima, S, Nishii, N, Kametani, Y, Shiina, T, Kulski, JK & Kitagawa, H 2019, 'Genetic Association between Swine Leukocyte antigen Class II Haplotypes and Reproduction Traits in Microminipigs' Cells, vol. 8, no. 8, 783. https://doi.org/10.3390/cells8080783

Genetic Association between Swine Leukocyte antigen Class II Haplotypes and Reproduction Traits in Microminipigs. / Ando, Asako; Imaeda, Noriaki; Matsubara, Tatsuya; Takasu, Masaki; Miyamoto, Asuka; Oshima, Shino; Nishii, Naohito; Kametani, Yoshie; Shiina, Takashi; Kulski, Jerzy K.; Kitagawa, Hitoshi.

In: Cells, Vol. 8, No. 8, 783, 08.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Genetic Association between Swine Leukocyte antigen Class II Haplotypes and Reproduction Traits in Microminipigs

AU - Ando, Asako

AU - Imaeda, Noriaki

AU - Matsubara, Tatsuya

AU - Takasu, Masaki

AU - Miyamoto, Asuka

AU - Oshima, Shino

AU - Nishii, Naohito

AU - Kametani, Yoshie

AU - Shiina, Takashi

AU - Kulski, Jerzy K.

AU - Kitagawa, Hitoshi

PY - 2019/8

Y1 - 2019/8

N2 - The effects of swine leukocyte antigen (SLA) molecules on numerous production and reproduction performance traits have been mainly reported as associations with specific SLA haplotypes that were assigned using serological typing methods. In this study, we intended to clarify the association between SLA class II genes and reproductive traits in a highly inbred population of 187 Microminipigs (MMP), that have eight different types of SLA class II haplotypes. In doing so, we compared the reproductive performances, such as fertility index, gestation period, litter size, and number of stillbirth among SLA class II low resolution haplotypes (Lrs) that were assigned by a polymerase chain reaction-sequence specific primers (PCR-SSP) typing method. Only low resolution haplotypes were used in this study because the eight SLA class II high-resolution haplotypes had been assigned to the 14 parents or the progenitors of the highly inbred MMP herd in a previous publication. The fertility index of dams with Lr-0.13 was significantly lower than that of dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. Dams with Lr-0.23 had significantly smaller litter size at birth than those with Lr-0.17, Lr-0.18, or Lr-0.37. Furthermore, litter size at weaning of dams with Lr-0.23 was also significantly smaller than those dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. The small litter size of dams with Lr-0.23 correlated with the smaller body sizes of these MMPs. These results suggest that SLA class II haplotypes are useful differential genetic markers for further haplotypic and epistatic studies of reproductive traits, selective breeding programs, and improvements in the production and reproduction performances of MMPs.

AB - The effects of swine leukocyte antigen (SLA) molecules on numerous production and reproduction performance traits have been mainly reported as associations with specific SLA haplotypes that were assigned using serological typing methods. In this study, we intended to clarify the association between SLA class II genes and reproductive traits in a highly inbred population of 187 Microminipigs (MMP), that have eight different types of SLA class II haplotypes. In doing so, we compared the reproductive performances, such as fertility index, gestation period, litter size, and number of stillbirth among SLA class II low resolution haplotypes (Lrs) that were assigned by a polymerase chain reaction-sequence specific primers (PCR-SSP) typing method. Only low resolution haplotypes were used in this study because the eight SLA class II high-resolution haplotypes had been assigned to the 14 parents or the progenitors of the highly inbred MMP herd in a previous publication. The fertility index of dams with Lr-0.13 was significantly lower than that of dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. Dams with Lr-0.23 had significantly smaller litter size at birth than those with Lr-0.17, Lr-0.18, or Lr-0.37. Furthermore, litter size at weaning of dams with Lr-0.23 was also significantly smaller than those dams with Lr-0.16, Lr-0.17, Lr-0.18, or Lr-0.37. The small litter size of dams with Lr-0.23 correlated with the smaller body sizes of these MMPs. These results suggest that SLA class II haplotypes are useful differential genetic markers for further haplotypic and epistatic studies of reproductive traits, selective breeding programs, and improvements in the production and reproduction performances of MMPs.

KW - swine leukocyte antigen

KW - reproductive performance

KW - production trait

KW - haplotype

KW - micro-mini-pigs

KW - MAJOR HISTOCOMPATIBILITY COMPLEX

KW - LITTER SIZE

KW - MOLECULAR CHARACTERIZATION

KW - HIGH-RESOLUTION

KW - SLA

KW - PIG

KW - SLA-DRB1

KW - SEQUENCE

KW - ALLELES

KW - GROWTH

U2 - 10.3390/cells8080783

DO - 10.3390/cells8080783

M3 - Article

VL - 8

JO - Cells

JF - Cells

SN - 2073-4409

IS - 8

M1 - 783

ER -