TY - JOUR
T1 - Genetic and molecular analysis of stem rot (Sclerotinia sclerotiorum) resistance in Brassica napus (canola type)
AU - Khan, Muhammad Azam
AU - Cowling, Wallace A.
AU - Banga, Surinder Singh
AU - Barbetti, Martin J.
AU - Cantila, Aldrin Y.
AU - Amas, Junrey C.
AU - Thomas, William J.W.
AU - You, Ming Pei
AU - Tyagi, Vikrant
AU - Bharti, Baudh
AU - Edwards, David
AU - Batley, Jacqueline
N1 - Funding Information:
The first author gratefully acknowledges a UWA-UAF Scholarship jointly from the University of Agriculture , Faisalabad (38000) in Pakistan and The University of Western Australia . The kind help and guidance in the molecular laboratories from Anita Severn-Ellis and Aneeta Pradhan is particularly appreciated. The authors gratefully acknowledge the exceptional technical support from Robert Creasy and Bill Piasini in the UWA Plant Growth Facilities. All authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. This research received no external funding with the components funded by the respective institutions.
Publisher Copyright:
© 2023 The Authors
PY - 2023/9
Y1 - 2023/9
N2 - Identifying the molecular and genetic basis of resistance to Sclerotinia stem rot (Sclerotinia sclerotiorum) is critical for developing long-term and cost-effective management of this disease in rapeseed/canola (Brassica napus). Current cultural or chemical management options provide, at best, only partial and/or sporadic control. Towards this, a B. napus breeding population (Mystic x Rainbow), including the parents, F1, F2, BC1P1 and BC1P2, was utilized in a field study to determine the inheritance pattern of Sclerotinia stem rot resistance (based on stem lesion length, SLL). Broad sense heritability was 0.58 for SLL and 0.44 for days to flowering (DTF). There was a significant negative correlation between SLL and stem diameter (SD) (r = −0.39) and between SLL and DTF (r = −0.28), suggesting co-selection of SD and DTF traits, along with SLL, should assist in improving overall resistance. Non-additive genetic variance was evident for SLL, DTF, and SD. In a genome wide association study (GWAS), a significant quantitative trait locus (QTL) was identified for SLL. Several putative candidate marker trait associations (MTA) were located within this QTL region. Overall, this study has provided valuable new understanding of inheritance of resistance to S. sclerotiorum, and has identified QTL, MTAs and transgressive segregants with high-level resistances. Together, these will foster more rapid selection for multiple traits associated with Sclerotinia stem rot resistance, by enabling breeders to make critical choices towards selecting/developing cultivars with enhanced resistance to this devastating pathogen.
AB - Identifying the molecular and genetic basis of resistance to Sclerotinia stem rot (Sclerotinia sclerotiorum) is critical for developing long-term and cost-effective management of this disease in rapeseed/canola (Brassica napus). Current cultural or chemical management options provide, at best, only partial and/or sporadic control. Towards this, a B. napus breeding population (Mystic x Rainbow), including the parents, F1, F2, BC1P1 and BC1P2, was utilized in a field study to determine the inheritance pattern of Sclerotinia stem rot resistance (based on stem lesion length, SLL). Broad sense heritability was 0.58 for SLL and 0.44 for days to flowering (DTF). There was a significant negative correlation between SLL and stem diameter (SD) (r = −0.39) and between SLL and DTF (r = −0.28), suggesting co-selection of SD and DTF traits, along with SLL, should assist in improving overall resistance. Non-additive genetic variance was evident for SLL, DTF, and SD. In a genome wide association study (GWAS), a significant quantitative trait locus (QTL) was identified for SLL. Several putative candidate marker trait associations (MTA) were located within this QTL region. Overall, this study has provided valuable new understanding of inheritance of resistance to S. sclerotiorum, and has identified QTL, MTAs and transgressive segregants with high-level resistances. Together, these will foster more rapid selection for multiple traits associated with Sclerotinia stem rot resistance, by enabling breeders to make critical choices towards selecting/developing cultivars with enhanced resistance to this devastating pathogen.
KW - Brassica QTL
KW - GWAS
KW - MTAs
KW - Sclerotinia
UR - http://www.scopus.com/inward/record.url?scp=85169452194&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2023.e19237
DO - 10.1016/j.heliyon.2023.e19237
M3 - Article
C2 - 37674843
AN - SCOPUS:85169452194
SN - 2405-8440
VL - 9
JO - Heliyon
JF - Heliyon
IS - 9
M1 - e19237
ER -