Genetic and Functional Analysis of R5X4 Human Immunodeficiency Virus Type 1 Envelope Glycoproteins Derived from Two Individuals Homozygous for the CCR5Δ32 Allele

L. Gray, M.J. Churchill, N. Keane, J. Sterjovski, A.M. Ellet, D.F.J. Purcell, P. Poumbourios, C. Kol, B. Wang, N.K. Saksena, S.L. Wesselingh, Patricia Price, Martyn French, D. Gabuzda, P.R. Gorry

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46 Citations (Web of Science)

Abstract

We characterized human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Env) isolated from two HIV-1-infected CCR5 Delta 32 homozygotes. Envs from both subjects used CCR5 and CXCR4 for entry into transfected cells. Most R5X4 Envs were lymphocyte-tropic and used CXCR4 exclusively for entry into peripheral blood mononuclear cells (PBMC), but a subset was dually lymphocyte- and macrophage-tropic and used either CCR5 or CXCR4 for entry into PBMC and monocyte-derived macrophages. The persistence of CCR5-using HIV-1 in two CCR5 Delta 32 homozygotes suggests the conserved CCR5 binding domain of Env is highly stable and provides new mechanistic insights important for HIV-1 transmission and persistence.
Original languageEnglish
Pages (from-to)3684-3691
JournalJournal of Virology
Volume80
Issue number7
DOIs
Publication statusPublished - 2006

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