Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum x P. fulvum interspecific cross

Oonagh Byrne, D.C. Hardie, Tanveer Khan, J. Speijers, Guijun Yan

    Research output: Contribution to journalArticle

    32 Citations (Scopus)

    Abstract

    Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F-2 population, with evidence of transgressive segregation. Heritability of pod resistance between F-2 and F-3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F-2 population by testing F-3 seed tissues of individual F-2 plants and pooling data from seed reaction for each F-2 plant (inferred F-2 genotype). Segregation for seed resistance in the F-2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F-2 to F-5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr(1), pwr(2), and pwr(3), and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (> 300 F-2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.
    Original languageEnglish
    Pages (from-to)854862
    JournalAustralian Journal of Agricultural Research
    Volume59
    DOIs
    Publication statusPublished - 2008

    Fingerprint

    Bruchus pisorum
    Pisum sativum
    genetic techniques and protocols
    pods
    seeds
    peas
    alleles
    inheritance (genetics)
    transgressive segregation
    backcrossing
    epistasis
    Pisum fulvum
    additive effect
    introgression
    heritability
    hybridization

    Cite this

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    title = "Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum x P. fulvum interspecific cross",
    abstract = "Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F-2 population, with evidence of transgressive segregation. Heritability of pod resistance between F-2 and F-3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F-2 population by testing F-3 seed tissues of individual F-2 plants and pooling data from seed reaction for each F-2 plant (inferred F-2 genotype). Segregation for seed resistance in the F-2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F-2 to F-5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr(1), pwr(2), and pwr(3), and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (> 300 F-2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.",
    author = "Oonagh Byrne and D.C. Hardie and Tanveer Khan and J. Speijers and Guijun Yan",
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    language = "English",
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    pages = "854862",
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    Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum x P. fulvum interspecific cross. / Byrne, Oonagh; Hardie, D.C.; Khan, Tanveer; Speijers, J.; Yan, Guijun.

    In: Australian Journal of Agricultural Research, Vol. 59, 2008, p. 854862.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Genetic analysis of pod and seed resistance to pea weevil in a Pisum sativum x P. fulvum interspecific cross

    AU - Byrne, Oonagh

    AU - Hardie, D.C.

    AU - Khan, Tanveer

    AU - Speijers, J.

    AU - Yan, Guijun

    PY - 2008

    Y1 - 2008

    N2 - Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F-2 population, with evidence of transgressive segregation. Heritability of pod resistance between F-2 and F-3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F-2 population by testing F-3 seed tissues of individual F-2 plants and pooling data from seed reaction for each F-2 plant (inferred F-2 genotype). Segregation for seed resistance in the F-2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F-2 to F-5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr(1), pwr(2), and pwr(3), and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (> 300 F-2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.

    AB - Interspecific populations derived from crossing cultivated field pea, Pisum sativum, with the wild pea relative, Pisum fulvum, were scored for pod and seed injury caused by the pea weevil, Bruchus pisorum. Pod resistance was quantitatively inherited in the F-2 population, with evidence of transgressive segregation. Heritability of pod resistance between F-2 and F-3 generations was very low, suggesting that this trait would be difficult to transfer in a breeding program. Seed resistance was determined for the F-2 population by testing F-3 seed tissues of individual F-2 plants and pooling data from seed reaction for each F-2 plant (inferred F-2 genotype). Segregation for seed resistance in the F-2 population of the cross Pennant/ATC113 showed a trigenic mode of inheritance, with additive effects and dominant epistasis towards susceptibility. Seed resistance was conserved over consecutive generations (F-2 to F-5) and successfully transferred to a new population by backcross introgression. Seed resistance in the backcross introgressed population segregated in a 63 : 1 ratio, supporting the three-gene inheritance model. It is proposed that complete resistance to pea weevil is controlled by three major recessive alleles assigned pwr(1), pwr(2), and pwr(3), and complete susceptibility by three major dominant alleles assigned PWR1, PWR2, and PWR3. It is recommended that large populations (> 300 F-2 plants) would be required to effectively transfer these recessive alleles to current field pea cultivars through hybridisation and repeated backcrossing.

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    JF - Crop & Pasture Science

    SN - 1836-0947

    ER -