Genetic analysis of cotyledon derived regenerants of tomato using AFLP markers

P. Bhatia; N. Ashwath; Tissa Senaratna; Siegfried Krauss

Genetic analysis of cotyledon derived regenerants of tomato using AFLP markers

P. Bhatia, N. Ashwath, Tissa Senaratna, Siegfried Krauss

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Abstract

MS medium supplemented with 15 muM zeatin was used for direct shoot regeneration from cotyledonary explants. DNA samples obtained from the regenerated shoots and parent cotyledonary explants were subjected to amplified fragment length polymorphism (AFLP) analysis to examine genetic uniformity of tissue-cultured tomato plants. Eighty-five markers were generated using the primer pair M-CAC/E-ACT and M-CTC/E-ACT. Identical fingerprints were generated for each primer pair for the mother and the regenerated plants. AFLP markers proved to be an ideal tool for routine analysis of genetic fidelity of regenerated tomato plants. This is a report on evaluating the genetic fidelity of tissue culture tomato plants using AFLP.

Original language	English
Pages (from-to)	280-284
Journal	Current Science
Volume	88
Issue number	2
Publication status	Published - 2005

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title = "Genetic analysis of cotyledon derived regenerants of tomato using AFLP markers",

abstract = "MS medium supplemented with 15 muM zeatin was used for direct shoot regeneration from cotyledonary explants. DNA samples obtained from the regenerated shoots and parent cotyledonary explants were subjected to amplified fragment length polymorphism (AFLP) analysis to examine genetic uniformity of tissue-cultured tomato plants. Eighty-five markers were generated using the primer pair M-CAC/E-ACT and M-CTC/E-ACT. Identical fingerprints were generated for each primer pair for the mother and the regenerated plants. AFLP markers proved to be an ideal tool for routine analysis of genetic fidelity of regenerated tomato plants. This is a report on evaluating the genetic fidelity of tissue culture tomato plants using AFLP.",

author = "P. Bhatia and N. Ashwath and Tissa Senaratna and Siegfried Krauss",

year = "2005",

language = "English",

volume = "88",

pages = "280--284",

journal = "Current Science",

issn = "0011-3891",

publisher = "Indian Academy of Sciences",

number = "2",

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TY - JOUR

T1 - Genetic analysis of cotyledon derived regenerants of tomato using AFLP markers

AU - Bhatia, P.

AU - Ashwath, N.

AU - Senaratna, Tissa

AU - Krauss, Siegfried

PY - 2005

Y1 - 2005

N2 - MS medium supplemented with 15 muM zeatin was used for direct shoot regeneration from cotyledonary explants. DNA samples obtained from the regenerated shoots and parent cotyledonary explants were subjected to amplified fragment length polymorphism (AFLP) analysis to examine genetic uniformity of tissue-cultured tomato plants. Eighty-five markers were generated using the primer pair M-CAC/E-ACT and M-CTC/E-ACT. Identical fingerprints were generated for each primer pair for the mother and the regenerated plants. AFLP markers proved to be an ideal tool for routine analysis of genetic fidelity of regenerated tomato plants. This is a report on evaluating the genetic fidelity of tissue culture tomato plants using AFLP.

AB - MS medium supplemented with 15 muM zeatin was used for direct shoot regeneration from cotyledonary explants. DNA samples obtained from the regenerated shoots and parent cotyledonary explants were subjected to amplified fragment length polymorphism (AFLP) analysis to examine genetic uniformity of tissue-cultured tomato plants. Eighty-five markers were generated using the primer pair M-CAC/E-ACT and M-CTC/E-ACT. Identical fingerprints were generated for each primer pair for the mother and the regenerated plants. AFLP markers proved to be an ideal tool for routine analysis of genetic fidelity of regenerated tomato plants. This is a report on evaluating the genetic fidelity of tissue culture tomato plants using AFLP.

M3 - Article

VL - 88

SP - 280

EP - 284

JO - Current Science

JF - Current Science

SN - 0011-3891

IS - 2

ER -

Genetic analysis of cotyledon derived regenerants of tomato using AFLP markers

Abstract

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