Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200

Dan Zhang; Samuel McLenachan; Shang Chih Chen; Khine Zaw; Yaqin Alziyadat; Xiao Zhang; Tina M. Lamey; Jennifer A. Thompson; Terri L. McLaren; Carla Mellough; John N. De Roach; Fred K. Chen

doi:10.1016/j.scr.2020.102154

Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200

Dan Zhang, Samuel McLenachan, Shang Chih Chen, Khine Zaw, Yaqin Alziyadat, Xiao Zhang, Tina M. Lamey, Jennifer A. Thompson, Terri L. McLaren, Carla Mellough, John N. De Roach, Fred K. Chen

Centre for Ophthalmology and Visual Science (affiliated with the Lions Eye Institute)

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The human induced pluripotent stem cell (iPSC) lines LEIi015-A and LEIi015-B were derived from a patient with inherited retinal disease caused by compound heterozygous mutations in the SNRNP200 gene (c.[1792C>T];[3341T>C]). Dermal fibroblasts were transfected with episomal plasmids carrying transgenes encoding OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for P53. The clonal iPSC lines LEIi015-A and LEIi015-B expressed iPSC markers, were free from genomic alterations and demonstrated trilineage differentiation potential.

Original language	English
Article number	102154
Journal	Stem Cell Research
Volume	51
DOIs	https://doi.org/10.1016/j.scr.2020.102154
Publication status	Published - Mar 2021

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.scr.2020.102154Licence: CC BY

Cite this

Zhang, D., McLenachan, S., Chen, S. C., Zaw, K., Alziyadat, Y., Zhang, X., Lamey, T. M., Thompson, J. A., McLaren, T. L., Mellough, C., De Roach, J. N., & Chen, F. K. (2021). Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200. Stem Cell Research, 51, Article 102154. https://doi.org/10.1016/j.scr.2020.102154

@article{6ba9643d64c944ed9ad502350aa1651c,

title = "Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200",

abstract = "The human induced pluripotent stem cell (iPSC) lines LEIi015-A and LEIi015-B were derived from a patient with inherited retinal disease caused by compound heterozygous mutations in the SNRNP200 gene (c.[1792C>T];[3341T>C]). Dermal fibroblasts were transfected with episomal plasmids carrying transgenes encoding OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for P53. The clonal iPSC lines LEIi015-A and LEIi015-B expressed iPSC markers, were free from genomic alterations and demonstrated trilineage differentiation potential.",

author = "Dan Zhang and Samuel McLenachan and Chen, {Shang Chih} and Khine Zaw and Yaqin Alziyadat and Xiao Zhang and Lamey, {Tina M.} and Thompson, {Jennifer A.} and McLaren, {Terri L.} and Carla Mellough and {De Roach}, {John N.} and Chen, {Fred K.}",

year = "2021",

month = mar,

doi = "10.1016/j.scr.2020.102154",

language = "English",

volume = "51",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Pergamon",

}

Zhang, D, McLenachan, S, Chen, SC, Zaw, K, Alziyadat, Y, Zhang, X, Lamey, TM, Thompson, JA, McLaren, TL, Mellough, C, De Roach, JN & Chen, FK 2021, 'Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200', Stem Cell Research, vol. 51, 102154. https://doi.org/10.1016/j.scr.2020.102154

TY - JOUR

T1 - Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200

AU - Zhang, Dan

AU - McLenachan, Samuel

AU - Chen, Shang Chih

AU - Zaw, Khine

AU - Alziyadat, Yaqin

AU - Zhang, Xiao

AU - Lamey, Tina M.

AU - Thompson, Jennifer A.

AU - McLaren, Terri L.

AU - Mellough, Carla

AU - De Roach, John N.

AU - Chen, Fred K.

PY - 2021/3

Y1 - 2021/3

N2 - The human induced pluripotent stem cell (iPSC) lines LEIi015-A and LEIi015-B were derived from a patient with inherited retinal disease caused by compound heterozygous mutations in the SNRNP200 gene (c.[1792C>T];[3341T>C]). Dermal fibroblasts were transfected with episomal plasmids carrying transgenes encoding OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for P53. The clonal iPSC lines LEIi015-A and LEIi015-B expressed iPSC markers, were free from genomic alterations and demonstrated trilineage differentiation potential.

AB - The human induced pluripotent stem cell (iPSC) lines LEIi015-A and LEIi015-B were derived from a patient with inherited retinal disease caused by compound heterozygous mutations in the SNRNP200 gene (c.[1792C>T];[3341T>C]). Dermal fibroblasts were transfected with episomal plasmids carrying transgenes encoding OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for P53. The clonal iPSC lines LEIi015-A and LEIi015-B expressed iPSC markers, were free from genomic alterations and demonstrated trilineage differentiation potential.

UR - http://www.scopus.com/inward/record.url?scp=85099019372&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2020.102154

DO - 10.1016/j.scr.2020.102154

M3 - Article

C2 - 33429167

AN - SCOPUS:85099019372

SN - 1873-5061

VL - 51

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102154

ER -

Generation of two induced pluripotent stem cell lines from a patient with recessive inherited retinal disease caused by compound heterozygous mutations in SNRNP200

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this