Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Samuel McLenachan; Elaine Y.M. Wong; Xiao Zhang; Fiona Leith; Sang Yoon Moon; Dan Zhang; Shang Chih Chen; Jennifer A. Thompson; Terri McLaren; Tina Lamey; John N. De Roach; Marcus D. Atlas; Rodney J. Dilley; Fred K. Chen

doi:10.1016/j.scr.2019.101420

Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Samuel McLenachan
, Elaine Y.M. Wong
, Xiao Zhang
, Fiona Leith
, Sang Yoon Moon
, Dan Zhang
, Shang Chih Chen
, Jennifer A. Thompson
, Terri McLaren
, Tina Lamey
, John N. De Roach
, Marcus D. Atlas
, Rodney J. Dilley
, Fred K. Chen

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.

Original language	English
Article number	101420
Number of pages	5
Journal	Stem Cell Research
Volume	36
DOIs	https://doi.org/10.1016/j.scr.2019.101420
Publication status	Published - 1 Apr 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1016/j.scr.2019.101420Licence: CC BY-NC-ND

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McLenachan, S., Wong, E. Y. M., Zhang, X., Leith, F., Moon, S. Y., Zhang, D., Chen, S. C., Thompson, J. A., McLaren, T., Lamey, T., De Roach, J. N., Atlas, M. D., Dilley, R. J., & Chen, F. K. (2019). Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene. Stem Cell Research, 36, Article 101420. https://doi.org/10.1016/j.scr.2019.101420

@article{9e8efe88d4bb4e2f8b2ae6881977ae2b,

title = "Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene",

abstract = "The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.",

author = "Samuel McLenachan and Wong, \{Elaine Y.M.\} and Xiao Zhang and Fiona Leith and Moon, \{Sang Yoon\} and Dan Zhang and Chen, \{Shang Chih\} and Thompson, \{Jennifer A.\} and Terri McLaren and Tina Lamey and \{De Roach\}, \{John N.\} and Atlas, \{Marcus D.\} and Dilley, \{Rodney J.\} and Chen, \{Fred K.\}",

year = "2019",

month = apr,

day = "1",

doi = "10.1016/j.scr.2019.101420",

language = "English",

volume = "36",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

McLenachan, S , Wong, EYM, Zhang, X, Leith, F, Moon, SY, Zhang, D, Chen, SC, Thompson, JA, McLaren, T, Lamey, T, De Roach, JN, Atlas, MD , Dilley, RJ & Chen, FK 2019, 'Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene', Stem Cell Research, vol. 36, 101420. https://doi.org/10.1016/j.scr.2019.101420

TY - JOUR

T1 - Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

AU - McLenachan, Samuel

AU - Wong, Elaine Y.M.

AU - Zhang, Xiao

AU - Leith, Fiona

AU - Moon, Sang Yoon

AU - Zhang, Dan

AU - Chen, Shang Chih

AU - Thompson, Jennifer A.

AU - McLaren, Terri

AU - Lamey, Tina

AU - De Roach, John N.

AU - Atlas, Marcus D.

AU - Dilley, Rodney J.

AU - Chen, Fred K.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.

AB - The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.

UR - https://www.scopus.com/pages/publications/85063079816

U2 - 10.1016/j.scr.2019.101420

DO - 10.1016/j.scr.2019.101420

M3 - Article

C2 - 30904819

AN - SCOPUS:85063079816

SN - 1873-5061

VL - 36

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101420

ER -

Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Abstract

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MRFF - Developing Personalised Treatment for Retinal Degeneration

From discovery to therapy in genetic eye diseases

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Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Abstract

UN SDGs

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Projects

MRFF - Developing Personalised Treatment for Retinal Degeneration

From discovery to therapy in genetic eye diseases

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