Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Samuel McLenachan; Elaine Y.M. Wong; Xiao Zhang; Fiona Leith; Sang Yoon Moon; Dan Zhang; Shang Chih Chen; Jennifer A. Thompson; Terri McLaren; Tina Lamey; John N. De Roach; Marcus D. Atlas; Rodney J. Dilley; Fred K. Chen

doi:10.1016/j.scr.2019.101420

Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Samuel McLenachan, Elaine Y.M. Wong, Xiao Zhang, Fiona Leith, Sang Yoon Moon, Dan Zhang, Shang Chih Chen, Jennifer A. Thompson, Terri McLaren, Tina Lamey, John N. De Roach, Marcus D. Atlas, Rodney J. Dilley, Fred K. Chen

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.

Original language	English
Article number	101420
Number of pages	5
Journal	Stem Cell Research
Volume	36
DOIs	https://doi.org/10.1016/j.scr.2019.101420
Publication status	Published - 1 Apr 2019

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10.1016/j.scr.2019.101420Licence: CC BY-NC-ND

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McLenachan, S., Wong, E. Y. M., Zhang, X., Leith, F., Moon, S. Y., Zhang, D., Chen, S. C., Thompson, J. A., McLaren, T., Lamey, T., De Roach, J. N., Atlas, M. D., Dilley, R. J., & Chen, F. K. (2019). Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene. Stem Cell Research, 36, Article 101420. https://doi.org/10.1016/j.scr.2019.101420

@article{9e8efe88d4bb4e2f8b2ae6881977ae2b,

title = "Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene",

abstract = "The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.",

author = "Samuel McLenachan and Wong, {Elaine Y.M.} and Xiao Zhang and Fiona Leith and Moon, {Sang Yoon} and Dan Zhang and Chen, {Shang Chih} and Thompson, {Jennifer A.} and Terri McLaren and Tina Lamey and {De Roach}, {John N.} and Atlas, {Marcus D.} and Dilley, {Rodney J.} and Chen, {Fred K.}",

year = "2019",

month = apr,

day = "1",

doi = "10.1016/j.scr.2019.101420",

language = "English",

volume = "36",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Pergamon",

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McLenachan, S , Wong, EYM, Zhang, X, Leith, F, Moon, SY, Zhang, D, Chen, SC, Thompson, JA, McLaren, T, Lamey, T, De Roach, JN, Atlas, MD , Dilley, RJ & Chen, FK 2019, 'Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene', Stem Cell Research, vol. 36, 101420. https://doi.org/10.1016/j.scr.2019.101420

TY - JOUR

T1 - Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

AU - McLenachan, Samuel

AU - Wong, Elaine Y.M.

AU - Zhang, Xiao

AU - Leith, Fiona

AU - Moon, Sang Yoon

AU - Zhang, Dan

AU - Chen, Shang Chih

AU - Thompson, Jennifer A.

AU - McLaren, Terri

AU - Lamey, Tina

AU - De Roach, John N.

AU - Atlas, Marcus D.

AU - Dilley, Rodney J.

AU - Chen, Fred K.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.

AB - The human iPSC lines LEIi010-A and LEIi010-B were generated from the dermal fibroblasts of a patient with Usher syndrome using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. These iPSC lines carry compound heterozygous mutations (c.949C > A and c.1256G > T) in USH2A. LEIi010-A and LEIi010-B expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages.

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U2 - 10.1016/j.scr.2019.101420

DO - 10.1016/j.scr.2019.101420

M3 - Article

C2 - 30904819

AN - SCOPUS:85063079816

SN - 1873-5061

VL - 36

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101420

ER -

Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Abstract

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MRFF - Developing Personalised Treatment for Retinal Degeneration

From discovery to therapy in genetic eye diseases

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Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

Abstract

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MRFF - Developing Personalised Treatment for Retinal Degeneration

From discovery to therapy in genetic eye diseases

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