Projects per year
Abstract
We report the generation of the human iPSC line LEIi007-A from a patient with autosomal recessive Stargardt disease caused by compound heterozygous mutations in the ABCA4 gene (c.[5461-10 T > C];[4139C > T]). Reprogramming of patient dermal fibroblasts was performed using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, shRNA for p53 and mir302/367 microRNA to establish the clonal iPSC line LEIl007-A. LEIl007-A displayed normal pluripotent stem cell colony morphology, expressed pluripotent stem cell markers, displayed a normal karyotype and differentiated into ectodermal, mesodermal and endodermal germ layer lineages.
Original language | English |
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Article number | 101352 |
Number of pages | 5 |
Journal | Stem Cell Research |
Volume | 34 |
Early online date | 30 Nov 2018 |
DOIs | |
Publication status | Published - Jan 2019 |
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Dive into the research topics of 'Generation of the induced pluripotent stem cell line from a patient with autosomal recessive ABCA4-mediated Stargardt Macular Dystrophy'. Together they form a unique fingerprint.Projects
- 2 Finished
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MRFF - Developing Personalised Treatment for Retinal Degeneration
National Health & Medical Research Council NHMRC
1/01/18 → 31/12/21
Project: Research
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From discovery to therapy in genetic eye diseases
Mackey, D., Craig, J., Hewitt, A., Burdon, K., Jamieson, R., Grigg, J., MacGregor, S., Chen, F., Otlowski, M. & Schofield, D.
National Health & Medical Research Council NHMRC
1/01/16 → 31/12/20
Project: Research