Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing

Peter J. Houweling; Vanessa Crossman; Chrystal F. Tiong; Chantal A. Coles; Rhonda L. Taylor; Joshua S. Clayton; Alison Graham; Katerina Vlahos; Sara E. Howden; Kathryn N. North

doi:10.1016/j.scr.2024.103313

Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing

Peter J. Houweling, Vanessa Crossman, Chrystal F. Tiong, Chantal A. Coles, Rhonda L. Taylor, Joshua S. Clayton, Alison Graham, Katerina Vlahos, Sara E. Howden, Kathryn N. North

Research output: Contribution to journal › Article › peer-review

Abstract

We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease.

Original language	English
Article number	103313
Number of pages	7
Journal	Stem Cell Research
Volume	75
Early online date	25 Jan 2024
DOIs	https://doi.org/10.1016/j.scr.2024.103313
Publication status	Published - Mar 2024

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title = "Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing",

abstract = "We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease.",

author = "Houweling, {Peter J.} and Vanessa Crossman and Tiong, {Chrystal F.} and Coles, {Chantal A.} and Taylor, {Rhonda L.} and Clayton, {Joshua S.} and Alison Graham and Katerina Vlahos and Howden, {Sara E.} and North, {Kathryn N.}",

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doi = "10.1016/j.scr.2024.103313",

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T1 - Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing

AU - Houweling, Peter J.

AU - Crossman, Vanessa

AU - Tiong, Chrystal F.

AU - Coles, Chantal A.

AU - Taylor, Rhonda L.

AU - Clayton, Joshua S.

AU - Graham, Alison

AU - Vlahos, Katerina

AU - Howden, Sara E.

AU - North, Kathryn N.

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N2 - We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease.

AB - We used gene editing to introduce DNA sequences encoding the tdTomato fluorescent protein into the α -skeletal actin 1 (ACTA1) locus to develop an ACTA1-tdTomato induced pluripotent stem cell reporter line for monitoring differentiation of skeletal muscle. This cell line will be used to better understand skeletal muscle maturation and development in vitro as well as provide a useful tool for drug screening and the evaluation of novel therapeutics for the treatment of skeletal muscle disease.

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DO - 10.1016/j.scr.2024.103313

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AN - SCOPUS:85183290628

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VL - 75

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 103313

ER -

Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing

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