TY - JOUR
T1 - Gel-free species identification using melt-curve analysis
AU - Berry, Oliver
AU - Sarre, S.D.
PY - 2007
Y1 - 2007
N2 - DNA-based identification of organisms is an important tool in biosecurity, ecological monitoring and wildlife forensics. Current methods usually involve post-polymerase chain reaction (PCR) manipulations (e.g. restriction digest, gel electrophoresis), which add to the expense and time required for processing samples, and may introduce error. We developed a method of species identification that uses species-specific primers and melt-curve analysis, and avoids post-PCR manipulation of samples. The method was highly accurate when trialled on DNA from six large carnivore species from Tasmania, Australia. Because of its flexibility and cost-effectiveness, this method should find wide use in many areas of applied biological science.
AB - DNA-based identification of organisms is an important tool in biosecurity, ecological monitoring and wildlife forensics. Current methods usually involve post-polymerase chain reaction (PCR) manipulations (e.g. restriction digest, gel electrophoresis), which add to the expense and time required for processing samples, and may introduce error. We developed a method of species identification that uses species-specific primers and melt-curve analysis, and avoids post-PCR manipulation of samples. The method was highly accurate when trialled on DNA from six large carnivore species from Tasmania, Australia. Because of its flexibility and cost-effectiveness, this method should find wide use in many areas of applied biological science.
U2 - 10.1111/j.1471-8286.2006.01541.x
DO - 10.1111/j.1471-8286.2006.01541.x
M3 - Article
SN - 1755-098X
VL - 7
SP - 1
EP - 4
JO - Molecular Ecology Resources
JF - Molecular Ecology Resources
IS - 1
ER -