Oxidant stress has been identified as important in the pathology of many diseases. Oxidation products of polyunsaturated fatty acids collectively termed isoprostanes, neuroprostanes, and isofurans are considered the most reliable measures of in vivo lipid oxidation, and they are widely used to assess oxidant stress in various diseases. Here we describe the measurement of these lipid oxidation products using gas chromatography mass spectrometry with electron capture negative ionization.
|Title of host publication||Clinical Metabolomics|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||10|
|Publication status||Published - 1 Jan 2018|
|Name||Methods in Molecular Biology|