Gastrin-induced gastric adenocarcinoma growth is mediated through cyclin D1

Diane H. Song, Basabi Rana, Jessica R. Wolfe, Geoffrey Crimmins, Caroline Choi, Chris Albanese, Timothy C. Wang, Richard G. Pestell, M. Michael Wolfe

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52 Citations (Scopus)


Gastrin is a gastrointestinal (GI) peptide that possesses potent trophic effects on most of the normal and neoplastic mucosa of the GI tract. Despite abundant evidence for these properties, the mechanisms governing gastrin-induced proliferation are still largely unknown. To elucidate the mechanisms by which gastrin might influence mitogenesis in gastric adenocarcinoma, we analyzed its effects on the human cell line AGS-B. Amidated gastrin (G-17), one of the major circulating forms of gastrin, induced a concentration-dependent increase in [3H]thymidine incorporation of cells in culture, with the maximum effective concentration occurring with 20 nM G-17. This effect was significantly attenuated by the gastrin-specific receptor antagonist L-365260. In addition, we found that G-17 induced a significant increase in the levels of cyclin D1 transcripts, protein, and promoter activity. The results of these studies indicate that gastrin appears to exert its mitogenic effects on gastric adenocarcinoma, at least in part, through changes in cyclin D1 expression.

Original languageEnglish
Pages (from-to)G217-G222
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number1 48-1
Publication statusPublished - 1 Jul 2003
Externally publishedYes


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