Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance

I Eujayl, William Erskine, B Bayaa, M Baum, E Pehu

    Research output: Contribution to journalArticle

    41 Citations (Scopus)

    Abstract

    The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp, lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692-16-1(s)) lines. F-2:4 progenies and F-6:8, F-6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well-established wilt-sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified For the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK-15(900)) at 10.8 cM. Two other RAPD markers (OP-B17(800) and OP-D15(500)) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP-C04(650)) was associated with repulsion. The DNA markers reported here will provide a starting point in marker-assisted selection for vascular wilt resistance in lentil.
    Original languageEnglish
    Pages (from-to)497-499
    JournalPlant Breeding
    Volume117
    DOIs
    Publication statusPublished - 1998

    Fingerprint

    vascular wilt
    Lens Plant
    Fusarium
    lentils
    Genetic Markers
    Blood Vessels
    inheritance (genetics)
    random amplified polymorphic DNA technique
    genetic markers
    Dominant Genes
    Lens culinaris
    dominant genes
    Fusarium oxysporum
    marker-assisted selection
    Vascular Resistance
    inbred lines
    Population
    linkage (genetics)
    loci

    Cite this

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    title = "Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance",
    abstract = "The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp, lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692-16-1(s)) lines. F-2:4 progenies and F-6:8, F-6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well-established wilt-sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified For the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK-15(900)) at 10.8 cM. Two other RAPD markers (OP-B17(800) and OP-D15(500)) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP-C04(650)) was associated with repulsion. The DNA markers reported here will provide a starting point in marker-assisted selection for vascular wilt resistance in lentil.",
    author = "I Eujayl and William Erskine and B Bayaa and M Baum and E Pehu",
    year = "1998",
    doi = "10.1111/j.1439-0523.1998.tb01982.x",
    language = "English",
    volume = "117",
    pages = "497--499",
    journal = "Plant Breeding",
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    publisher = "Wiley-Blackwell",

    }

    Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance. / Eujayl, I; Erskine, William; Bayaa, B; Baum, M; Pehu, E.

    In: Plant Breeding, Vol. 117, 1998, p. 497-499.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance

    AU - Eujayl, I

    AU - Erskine, William

    AU - Bayaa, B

    AU - Baum, M

    AU - Pehu, E

    PY - 1998

    Y1 - 1998

    N2 - The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp, lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692-16-1(s)) lines. F-2:4 progenies and F-6:8, F-6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well-established wilt-sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified For the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK-15(900)) at 10.8 cM. Two other RAPD markers (OP-B17(800) and OP-D15(500)) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP-C04(650)) was associated with repulsion. The DNA markers reported here will provide a starting point in marker-assisted selection for vascular wilt resistance in lentil.

    AB - The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp, lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692-16-1(s)) lines. F-2:4 progenies and F-6:8, F-6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well-established wilt-sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified For the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK-15(900)) at 10.8 cM. Two other RAPD markers (OP-B17(800) and OP-D15(500)) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP-C04(650)) was associated with repulsion. The DNA markers reported here will provide a starting point in marker-assisted selection for vascular wilt resistance in lentil.

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    DO - 10.1111/j.1439-0523.1998.tb01982.x

    M3 - Article

    VL - 117

    SP - 497

    EP - 499

    JO - Plant Breeding

    JF - Plant Breeding

    SN - 0179-9541

    ER -