Functional effects of polymorphisms of house dust mite allergens

W.R. Thomas, Wendy-Anne Smith, Belinda Hales, M.D. Carter

Research output: Contribution to journalReview article

22 Citations (Scopus)

Abstract

Background: Sequence polymorphisms have been found in cDNA clones of commercial house dust mites. Here we report on the polymorphisms of Der p 1 found in environmental mites and the effect of the polymorphisms on T cell proliferation. Methods: Sequences were obtained from DNA amplified from single mites by PCR. Synthetic peptides were used to stimulate peripheral blood cells. Results: The polymorphism was limited to a few residues, but regional differences were detected in mites in Wagga. T cells were specific for the polymorphisms, so that in 16 of 17 responses, stimulation was achieved with one form of the peptide. This included peptides with Ala and Val at position 124 in the immunodominant 110-133 region. Peptide 45-63 also stimulated frequently, but only with Tyr at position 50 and not His, a residue only found in commercial mites. Conclusions: Polymorphisms of Der p 1 are found in the environment and have a profound effect on polyclonal T cell stimulation to frequently recognised epitopes.
Original languageEnglish
Pages (from-to)96-98
JournalInternational Archives of Allergy and Immunology
Volume113
DOIs
Publication statusPublished - 1997

Fingerprint

Dive into the research topics of 'Functional effects of polymorphisms of house dust mite allergens'. Together they form a unique fingerprint.

Cite this