Folate-targeting redox hyperbranched poly(amido amine)s delivering MMP-9 siRNA for cancer therapy

M. Li; X. Zhou; X. Zeng; C. Wang; Jiake Xu; D. Ma; W. Xue

doi:10.1039/c5tb01964h

Folate-targeting redox hyperbranched poly(amido amine)s delivering MMP-9 siRNA for cancer therapy

M. Li, X. Zhou, X. Zeng, C. Wang, Jiake Xu, D. Ma, W. Xue

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

© The Royal Society of Chemistry 2016. For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N,N′-cystaminebisacrylamide. Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched poly(amido amine)s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than PEI-25k, suggesting a potential application in gene therapy for tumors.

Original language	English
Pages (from-to)	547-556
Number of pages	10
Journal	Journal of Materials Chemistry B
Volume	4
Issue number	3
DOIs	https://doi.org/10.1039/c5tb01964h
Publication status	Published - 2016

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title = "Folate-targeting redox hyperbranched poly(amido amine)s delivering MMP-9 siRNA for cancer therapy",

abstract = "{\textcopyright} The Royal Society of Chemistry 2016. For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N,N′-cystaminebisacrylamide. Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched poly(amido amine)s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than PEI-25k, suggesting a potential application in gene therapy for tumors.",

author = "M. Li and X. Zhou and X. Zeng and C. Wang and Jiake Xu and D. Ma and W. Xue",

year = "2016",

doi = "10.1039/c5tb01964h",

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T1 - Folate-targeting redox hyperbranched poly(amido amine)s delivering MMP-9 siRNA for cancer therapy

AU - Li, M.

AU - Zhou, X.

AU - Zeng, X.

AU - Wang, C.

AU - Xu, Jiake

AU - Ma, D.

AU - Xue, W.

PY - 2016

Y1 - 2016

N2 - © The Royal Society of Chemistry 2016. For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N,N′-cystaminebisacrylamide. Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched poly(amido amine)s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than PEI-25k, suggesting a potential application in gene therapy for tumors.

AB - © The Royal Society of Chemistry 2016. For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N,N′-cystaminebisacrylamide. Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched poly(amido amine)s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than PEI-25k, suggesting a potential application in gene therapy for tumors.

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U2 - 10.1039/c5tb01964h

DO - 10.1039/c5tb01964h

M3 - Article

SN - 2050-7518

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JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 3

ER -

Folate-targeting redox hyperbranched poly(amido amine)s delivering MMP-9 siRNA for cancer therapy

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