Folate-targeting redox hyperbranched poly(amido amine)s delivering MMP-9 siRNA for cancer therapy

M. Li, X. Zhou, X. Zeng, C. Wang, Jiake Xu, D. Ma, W. Xue

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

© The Royal Society of Chemistry 2016. For effective gene delivery to breast cancer MCF-7 cells, a folate-targeting redox gene carrier was synthesized by Michael addition polymerization between 1-(2-aminoethyl)piperazine and N,N′-cystaminebisacrylamide. Folate was then conjugated through an amidation reaction. The obtained folate-modified hyperbranched poly(amido amine)s (FA-PAAs) degraded in the presence of glutathione and displayed excellent transfection efficiency in vitro. In particular, FA-PAAs showed much higher gene delivery efficiency than PEI-25k in the presence of serum, leading to an obvious decrease in MMP-9 protein expression and the apoptosis of MCF-7 cells. Moreover, FA-PAAs displayed lower cytotoxicity and better blood compatibility than PEI-25k, suggesting a potential application in gene therapy for tumors.
Original languageEnglish
Pages (from-to)547-556
Number of pages10
JournalJournal of Materials Chemistry B
Volume4
Issue number3
DOIs
Publication statusPublished - 2016

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