Focused transcription from the human CR2/CD21 core promoter is regulated by synergistic activity of TATA and Initiator elements in mature B cells

Rhonda Taylor, Mark Cruickshank, Mahdad Karimi, Hans Ng, Elizabeth Quail, K.M. Kaufman, J.B. Harley, Lawrence Abraham, B.P. Tsao, S.A. Boackle, Daniela Ulgiati

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Complement receptor 2 (CR2/CD21) is predominantly expressed on the surface of mature B cells where it forms part of a coreceptor complex that functions, in part, to modulate B-cell receptor signal strength. CR2/CD21 expression is tightly regulated throughout B-cell development such that CR2/CD21 cannot be detected on pre-B or terminally differentiated plasma cells. CR2/CD21 expression is upregulated at B-cell maturation and can be induced by IL-4 and CD40 signaling pathways. We have previously characterized elements in the proximal promoter and first intron of CR2/CD21 that are involved in regulating basal and tissue-specific expression. We now extend these analyses to the CR2/CD21 core promoter. We show that in mature B cells, CR2/CD21 transcription proceeds from a focused TSS regulated by a non-consensus TATA box, an initiator element and a downstream promoter element. Furthermore, occupancy of the general transcriptional machinery in pre-B versus mature B-cell lines correlate with CR2/CD21 expression level and indicate that promoter accessibility must switch from inactive to active during the transitional B-cell window.
Original languageEnglish
Pages (from-to)119-131
Number of pages13
JournalCELLULAR & MOLECULAR IMMUNOLOGY
Volume13
Issue number1
Early online date2 Feb 2015
DOIs
Publication statusPublished - Jan 2016

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