Fluorescent protein tagging as a tool to define the subcellular distribution of proteins in plants

Sandra Tanz, I.R. Castleden, I.D. Small, Harvey Millar

Research output: Contribution to journalArticle

28 Citations (Scopus)
203 Downloads (Pure)

Abstract

Fluorescent protein (FP) tagging approaches are widely used to determine the subcellular location of plant proteins. Here we give a brief overview of FP approaches, highlight potential technical problems, and discuss what to consider when designing FP/protein fusion constructs and performing transformation assays. We analyze published FP tagging data sets along with data from proteomics studies collated in SUBA3, a subcellular location database for Arabidopsis proteins, and assess the reliability of these data sets by comparing them. We also outline the limitations of the FP tagging approach for defining protein location and investigate multiple localization claims by FP tagging. We conclude that the collation of localization datasets in databases like SUBA3 is helpful for revealing discrepancies in location attributions by different techniques and/or by different research groups. © 2013 Tanz, Castleden, Small and Millar.
Original languageEnglish
Pages (from-to)9pp
JournalFrontiers in Plant Science
Volume4
Issue numberJUN
DOIs
Publication statusPublished - 2013

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