TY - CHAP
T1 - Fluorescence lifetime imaging
AU - Suhling, Klaus
AU - Hirvonen, Liisa M.
AU - Levitt, James A.
AU - Chung, Pei Hua
AU - Tregidgo, Carolyn
AU - Rusakov, Dmitri A.
AU - Zheng, Kaiyu
AU - Ameer-Beg, Simon
AU - Poland, Simon P.
AU - Coelho, Simao
AU - Henderson, Robert
AU - Krstajic, Nikola
PY - 2017/3/29
Y1 - 2017/3/29
N2 - Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation intensity. Many FLIM applications relevant for biology concern the identification of Förster resonance energy transfer (FRET) to study protein interactions and conformational changes. In addition, FLIM has been used to image viscosity, temperature, pH, refractive index, and ion and oxygen concentrations, all at the cellular level. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, molecular probe, and FLIM detector development will be discussed.
AB - Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation intensity. Many FLIM applications relevant for biology concern the identification of Förster resonance energy transfer (FRET) to study protein interactions and conformational changes. In addition, FLIM has been used to image viscosity, temperature, pH, refractive index, and ion and oxygen concentrations, all at the cellular level. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, molecular probe, and FLIM detector development will be discussed.
KW - Anisotropy
KW - Fluorescence anisotropy imaging (FAIM)
KW - Fluorescence enhancement
KW - Fluorescence microscopy
KW - Fluorescence spectroscopy
KW - Förster resonance energy transfer (FRET)
KW - Plasmonics
KW - Time-correlated single-photon counting (TCSPC)
KW - Time-resolved fluorescence anisotropy imaging (TR-FAIM)
KW - Total internal reflection fluorescence (TIRF)
UR - https://www.scopus.com/pages/publications/85034393737
U2 - 10.1007/978-94-007-5052-4_13
DO - 10.1007/978-94-007-5052-4_13
M3 - Chapter
AN - SCOPUS:85034393737
SN - 9789400750517
SP - 353
EP - 405
BT - Handbook of Photonics for Biomedical Engineering
PB - Springer, Dordrecht, Netherlands
ER -