Fluorescence lifetime imaging (FLIM) measurements in salinity research

Olga Babourina, Zed Rengel

Research output: Chapter in Book/Conference paperChapterpeer-review

Abstract

Plant response to salt stress involves changes in intracellular ion concentrations that can be estimated by ion-selective fluorescent dyes. Conventional confocal/fluorescent imaging does not always accurately reflect the process, since it is dependent not only on the ion concentration but on the dye concentration itself. Fluorescent lifetime imaging (FLIM) can resolve this problem: images derived under this technique are based on the fluorescent lifetime distribution of the bound/unbound state of the fluorophore, and not on the fluorescent intensity. On the example of 2′-7′-bis(carboxyethyl) -5(6)-carboxyfluorescein (BCECF) and Ca Green, we demonstrate the potential of FLIM technique in salinity studies.

Original languageEnglish
Title of host publicationPlant Salt Tolerance
Subtitle of host publicationMethods and Protocols
EditorsSergey Shabala, Tracey Ann Cuin
Place of PublicationUnited States
PublisherHumana Press Inc.
Pages149-161
Number of pages13
Edition1
ISBN (Electronic)9781617799860
ISBN (Print)9781617799853, 9781493959396
DOIs
Publication statusPublished - 16 Aug 2012

Publication series

NameMethods in Molecular Biology
Volume913
ISSN (Print)1064-3745

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