TY - JOUR
T1 - Fluorescence lifetime imaging (FLIM)
T2 - Basic concepts and some recent developments
AU - Suhling, Klaus
AU - Hirvonen, Liisa M.
AU - Levitt, James A.
AU - Chung, Pei Hua
AU - Tregidgo, Carolyn
AU - Le Marois, Alix
AU - Rusakov, Dmitri A.
AU - Zheng, Kaiyu
AU - Ameer-Beg, Simon
AU - Poland, Simon
AU - Coelho, Simao
AU - Henderson, Robert
AU - Krstajic, Nikola
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. One prominent FLIM application relevant for biological concerns is the identification of FRET to study protein interactions and conformational changes, but FLIM is also used to image viscosity, temperature, pH, refractive index and ion and oxygen concentrations, all at the cellular level, as well as cell and tissue autofluorescence. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, data analysis, molecular probe and FLIM detector development will be discussed.
AB - Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. One prominent FLIM application relevant for biological concerns is the identification of FRET to study protein interactions and conformational changes, but FLIM is also used to image viscosity, temperature, pH, refractive index and ion and oxygen concentrations, all at the cellular level, as well as cell and tissue autofluorescence. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, data analysis, molecular probe and FLIM detector development will be discussed.
KW - Fluorescence microscopy
KW - Fluorescence sensing
KW - Fluorescence spectroscopy
KW - Förster resonance energy transfer (FRET)
KW - Time-correlated single photon counting (TCSPC)
KW - Time-resolved fluorescence anisotropy imaging (TR-FAIM)
UR - http://www.scopus.com/inward/record.url?scp=84977120306&partnerID=8YFLogxK
U2 - 10.1016/j.medpho.2014.12.001
DO - 10.1016/j.medpho.2014.12.001
M3 - Review article
AN - SCOPUS:84977120306
VL - 27
SP - 3
EP - 40
JO - Medical Photonics
JF - Medical Photonics
SN - 2213-8846
ER -