Fluorescence lifetime imaging (Flim): Basic concepts and recent applications

Klaus Suhling; Liisa M. Hirvonen; James A. Levitt; Pei Hua Chung; Carolyn Tregido; Alix Le Marois; Dmitri A. Rusakov; Kaiyu Zheng; Simon Ameer-Beg; Simon Poland; Simon Coelho; Richard Dimble

doi:10.1007/978-3-319-14929-5_3

Fluorescence lifetime imaging (Flim): Basic concepts and recent applications

Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei Hua Chung, Carolyn Tregido, Alix Le Marois, Dmitri A. Rusakov, Kaiyu Zheng, Simon Ameer-Beg, Simon Poland, Simon Coelho, Richard Dimble

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photo physical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. A FLIM application relevant for biology concerns the identification of FRET to study protein interactions and conformational changes, and FLIM can also be used to image viscosity.

Original language	English
Pages (from-to)	119-188
Number of pages	70
Journal	Springer Series in Chemical Physics
Volume	111
DOIs	https://doi.org/10.1007/978-3-319-14929-5_3
Publication status	Published - 1 Jan 2015
Externally published	Yes

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10.1007/978-3-319-14929-5_3

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title = "Fluorescence lifetime imaging (Flim): Basic concepts and recent applications",

abstract = "Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photo physical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. A FLIM application relevant for biology concerns the identification of FRET to study protein interactions and conformational changes, and FLIM can also be used to image viscosity.",

author = "Klaus Suhling and Hirvonen, {Liisa M.} and Levitt, {James A.} and Chung, {Pei Hua} and Carolyn Tregido and Marois, {Alix Le} and Rusakov, {Dmitri A.} and Kaiyu Zheng and Simon Ameer-Beg and Simon Poland and Simon Coelho and Richard Dimble",

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doi = "10.1007/978-3-319-14929-5_3",

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T2 - Basic concepts and recent applications

AU - Suhling, Klaus

AU - Hirvonen, Liisa M.

AU - Levitt, James A.

AU - Chung, Pei Hua

AU - Tregido, Carolyn

AU - Marois, Alix Le

AU - Rusakov, Dmitri A.

AU - Zheng, Kaiyu

AU - Ameer-Beg, Simon

AU - Poland, Simon

AU - Coelho, Simon

AU - Dimble, Richard

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photo physical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. A FLIM application relevant for biology concerns the identification of FRET to study protein interactions and conformational changes, and FLIM can also be used to image viscosity.

AB - Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photo physical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. A FLIM application relevant for biology concerns the identification of FRET to study protein interactions and conformational changes, and FLIM can also be used to image viscosity.

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U2 - 10.1007/978-3-319-14929-5_3

DO - 10.1007/978-3-319-14929-5_3

M3 - Article

AN - SCOPUS:84927917200

SN - 0172-6218

VL - 111

SP - 119

EP - 188

JO - Springer Series in Chemical Physics

JF - Springer Series in Chemical Physics

ER -

Fluorescence lifetime imaging (Flim): Basic concepts and recent applications

Abstract

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