@article{caf684e0843246c3bf4e838da341c084,
title = "Flow cytometric characterization of tissue-resident lymphocytes after murine liver and heart transplantation",
abstract = "Alterations to organ biology caused by transplantation can have major impacts on the outcome. Tissue-resident lymphocytes normally maintain an organ's immunity and function and are transferred during transplantation. Here, we provide a detailed protocol for the isolation of leukocytes, including tissue-resident lymphocytes, from transplanted livers and hearts in mice. Phenotypic and functional analysis of conventional and unconventional T cells by flow cytometry is included. This protocol can also be used for the effective isolation of leukocytes from non-transplanted livers and hearts. For complete details on the use and execution of this protocol, please refer to Prosser et al. (2021).",
keywords = "Cell isolation, Flow Cytometry/Mass Cytometry, Immunology",
author = "Amy Prosser and Sarah Dart and Irma Larma-Cornwall and Michaela Lucas",
note = "Funding Information: The authors acknowledge the facilities and assistance of the Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, a facility funded by the University, State, and Commonwealth governments. We also thank Ms. Liu Liu, Dr. Wen Hua Huang, and Professor Axel Kallies for their technical expertise and the staff of The University of Western Australia{\textquoteright}s Animal Care Services and Dr. Alison McDonnell for their assistance. This work was supported by the Western Australian Department of Health and the Sir Charles Gairdner and Osborne Park Health Care Group Research Advisory Committee. A.P. was supported by an Australian Government Research Training Program Scholarship. The graphical abstract and figures were created with BioRender.com . Funding Information: The authors acknowledge the facilities and assistance of the Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, a facility funded by the University, State, and Commonwealth governments. We also thank Ms. Liu Liu, Dr. Wen Hua Huang, and Professor Axel Kallies for their technical expertise and the staff of The University of Western Australia's Animal Care Services and Dr. Alison McDonnell for their assistance. This work was supported by the Western Australian Department of Health and the Sir Charles Gairdner and Osborne Park Health Care Group Research Advisory Committee. A.P. was supported by an Australian Government Research Training Program Scholarship. The graphical abstract and figures were created with BioRender.com. Conceptualization, A.P. and M.L.; methodology, A.P. I.L.-C. and S.D.; investigation, A.P. and S.D.; data curation, A.P. and S.D.; writing ? original draft, A.P.; writing ? review and editing, A.P. S.D. and M.L.; funding acquisition, A.P. and M.L. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = dec,
day = "17",
doi = "10.1016/j.xpro.2021.100810",
language = "English",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",
}