Flow cytometric characterization of tissue-resident lymphocytes after murine liver and heart transplantation

Amy Prosser, Sarah Dart, Irma Larma-Cornwall, Michaela Lucas

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Alterations to organ biology caused by transplantation can have major impacts on the outcome. Tissue-resident lymphocytes normally maintain an organ's immunity and function and are transferred during transplantation. Here, we provide a detailed protocol for the isolation of leukocytes, including tissue-resident lymphocytes, from transplanted livers and hearts in mice. Phenotypic and functional analysis of conventional and unconventional T cells by flow cytometry is included. This protocol can also be used for the effective isolation of leukocytes from non-transplanted livers and hearts. For complete details on the use and execution of this protocol, please refer to Prosser et al. (2021).

Original languageEnglish
Article number100810
JournalSTAR Protocols
Volume2
Issue number4
DOIs
Publication statusPublished - 17 Dec 2021

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