Flavonoids are involved in phosphorus-deficiency-induced cluster-root formation in white lupin

Chuanyong Xiong, Xiaoqing Li, Xin Wang, Jingxin Wang, Hans Lambers, Carroll P. Vance, Jianbo Shen, Lingyun Cheng

Research output: Contribution to journalArticlepeer-review

1 Citation (Web of Science)

Abstract

BACKGROUND AND AIMS: Initiation of cluster roots in white lupin (Lupinus albus) under phosphorus (P) deficiency requires auxin signalling, whereas flavonoids inhibit auxin transport. However, little information is available about the interactions between P deficiency and flavonoids in terms of cluster-root formation in white lupin. METHODS: Hydroponic and aeroponic systems were used to investigate the role of flavonoids in cluster-root formation, with or without 75 μm P supply. KEY RESULTS: Phosphorus-deficiency-induced flavonoid accumulation in cluster roots depended on developmental stage, based on in situ determination of fluorescence of flavonoids and flavonoid concentration. LaCHS8, which codes for a chalcone synthase isoform, was highly expressed in cluster roots, and silencing LaCHS8 reduced flavonoid production and rootlet density. Exogenous flavonoids suppressed cluster-root formation. Tissue-specific distribution of flavonoids in roots was altered by P deficiency, suggesting that P deficiency induced flavonoid accumulation, thus fine-tuning the effect of flavonoids on cluster-root formation. Furthermore, naringenin inhibited expression of an auxin-responsive DR5:GUS marker, suggesting an interaction of flavonoids and auxin in regulating cluster-root formation. CONCLUSIONS: Phosphorus deficiency triggered cluster-root formation through the regulation of flavonoid distribution, which fine-tuned an auxin response in the early stages of cluster-root development. These findings provide valuable insights into the mechanisms of cluster-root formation under P deficiency.

Original languageEnglish
Pages (from-to)101-112
Number of pages12
JournalAnnals of Botany
Volume129
Issue number1
DOIs
Publication statusPublished - 8 Jan 2022

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