Fingerprinting of cauliflower cultivars using RAPD markers

I.A. Astarini; Julie Plummer; R.A. Lancaster; Guijun Yan

doi:10.1071/AR03012

Fingerprinting of cauliflower cultivars using RAPD markers

I.A. Astarini, Julie Plummer, R.A. Lancaster, Guijun Yan

Graduate Research School

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.

Original language	English
Pages (from-to)	117-124
Journal	Australian Journal of Agricultural Research
Volume	55
Issue number	2
DOIs	https://doi.org/10.1071/AR03012
Publication status	Published - 2004

Access to Document

10.1071/AR03012

Cite this

@article{f19895be8d1c4cf8b08de04047de7f68,

title = "Fingerprinting of cauliflower cultivars using RAPD markers",

abstract = "Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.",

author = "I.A. Astarini and Julie Plummer and R.A. Lancaster and Guijun Yan",

year = "2004",

doi = "10.1071/AR03012",

language = "English",

volume = "55",

pages = "117--124",

journal = " Australian Journal of Agricultural Research",

issn = "0004-9409",

publisher = "CSIRO Publishing",

number = "2",

}

TY - JOUR

T1 - Fingerprinting of cauliflower cultivars using RAPD markers

AU - Astarini, I.A.

AU - Plummer, Julie

AU - Lancaster, R.A.

AU - Yan, Guijun

PY - 2004

Y1 - 2004

N2 - Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.

AB - Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.

U2 - 10.1071/AR03012

DO - 10.1071/AR03012

M3 - Article

SN - 0004-9409

VL - 55

SP - 117

EP - 124

JO - Australian Journal of Agricultural Research

JF - Australian Journal of Agricultural Research

IS - 2

ER -