Fingerprinting of cauliflower cultivars using RAPD markers

I.A. Astarini, Julie Plummer, R.A. Lancaster, Guijun Yan

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Randomly amplified polymorphic DNA (RAPD) was used to investigate genetic relationships among 25 cultivars of cauliflower (Brassica oleracea var. botrytis L.). Forty decamer primers were examined, among which 15 primers produced polymorphism. Twenty-five polymorphic bands were observed, ranging in size from 428 to 1646 bp. A fingerprinting key was generated using these polymorphic bands. A dendogram was constructed using neighbour-joining analysis based on phylogenetic analysis using parsimony (PAUP). Results indicate that RAPD markers can be used for the routine identification of cauliflower cultivars within B. oleracea var. botrytis L.
Original languageEnglish
Pages (from-to)117-124
Journal Australian Journal of Agricultural Research
Volume55
Issue number2
DOIs
Publication statusPublished - 2004

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