Field trials of blood culture identification FilmArray in regional Australian hospitals

Nicole M. Bzdyl, Nadezda Urosevic, Ben Payne, Ray Brockenshire, Michael McIntyre, Michael J. Leung, Graham Weaire-Buchanan, Elizabeth Geelhoed, Timothy J J Inglis

Research output: Contribution to journalArticle

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Abstract

PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories.

METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure.

RESULTS: Of 100 positive blood culture episodes, BCID FilmArray results were correct in all 42 significant monobacterial cultures, with a fully predictive identity in 38 (90.5 %) and a partial identity in another four (9.5 %). There was one major error; a false positive Pseudomonas aeruginosa. The minor errors were the detection of one methicillin-resistant Staphylococcus aureus, which proved to be a methicillin-sensitive S. aureus mixed with a methicillin-resistant coagulase-negative staphylococcus, five false negative coagulase-negative staphylococci and one false negative streptococcus species. We found that 41/49 (84 %) clinically significant mono- and polymicrobial culture results were fully predictive of culture-based identification to bacterial species level at a mean of 1.15 days after specimen collection.

CONCLUSIONS: There was a reduction of 1.21 days in the time taken to produce a definitive BCID compared to the previous year, translating into earlier communication of more specific blood culture results to the treating physician. Reduced time to definitive blood culture results has a direct benefit for isolated Australian communities at great distances from specialist hospital services.

Original languageEnglish
Article number000714
Pages (from-to)669-675
JournalJournal of Medical Microbiology
Volume67
Issue number5
DOIs
Publication statusE-pub ahead of print - 13 Mar 2018

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Coagulase
Multiplex Polymerase Chain Reaction
Staphylococcus
Specimen Handling
Hospital Laboratories
Methicillin Resistance
Methicillin
Workflow
Blood Culture
Methicillin-Resistant Staphylococcus aureus
Streptococcus
Pseudomonas aeruginosa
France
Staphylococcus aureus
Communication
Physicians
Equipment and Supplies

Cite this

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title = "Field trials of blood culture identification FilmArray in regional Australian hospitals",
abstract = "PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories.METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure.RESULTS: Of 100 positive blood culture episodes, BCID FilmArray results were correct in all 42 significant monobacterial cultures, with a fully predictive identity in 38 (90.5 {\%}) and a partial identity in another four (9.5 {\%}). There was one major error; a false positive Pseudomonas aeruginosa. The minor errors were the detection of one methicillin-resistant Staphylococcus aureus, which proved to be a methicillin-sensitive S. aureus mixed with a methicillin-resistant coagulase-negative staphylococcus, five false negative coagulase-negative staphylococci and one false negative streptococcus species. We found that 41/49 (84 {\%}) clinically significant mono- and polymicrobial culture results were fully predictive of culture-based identification to bacterial species level at a mean of 1.15 days after specimen collection.CONCLUSIONS: There was a reduction of 1.21 days in the time taken to produce a definitive BCID compared to the previous year, translating into earlier communication of more specific blood culture results to the treating physician. Reduced time to definitive blood culture results has a direct benefit for isolated Australian communities at great distances from specialist hospital services.",
author = "Bzdyl, {Nicole M.} and Nadezda Urosevic and Ben Payne and Ray Brockenshire and Michael McIntyre and Leung, {Michael J.} and Graham Weaire-Buchanan and Elizabeth Geelhoed and Inglis, {Timothy J J}",
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Field trials of blood culture identification FilmArray in regional Australian hospitals. / Bzdyl, Nicole M.; Urosevic, Nadezda; Payne, Ben; Brockenshire, Ray; McIntyre, Michael; Leung, Michael J.; Weaire-Buchanan, Graham; Geelhoed, Elizabeth; Inglis, Timothy J J.

In: Journal of Medical Microbiology, Vol. 67, No. 5, 000714, 13.03.2018, p. 669-675.

Research output: Contribution to journalArticle

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AU - Bzdyl, Nicole M.

AU - Urosevic, Nadezda

AU - Payne, Ben

AU - Brockenshire, Ray

AU - McIntyre, Michael

AU - Leung, Michael J.

AU - Weaire-Buchanan, Graham

AU - Geelhoed, Elizabeth

AU - Inglis, Timothy J J

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Y1 - 2018/3/13

N2 - PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories.METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure.RESULTS: Of 100 positive blood culture episodes, BCID FilmArray results were correct in all 42 significant monobacterial cultures, with a fully predictive identity in 38 (90.5 %) and a partial identity in another four (9.5 %). There was one major error; a false positive Pseudomonas aeruginosa. The minor errors were the detection of one methicillin-resistant Staphylococcus aureus, which proved to be a methicillin-sensitive S. aureus mixed with a methicillin-resistant coagulase-negative staphylococcus, five false negative coagulase-negative staphylococci and one false negative streptococcus species. We found that 41/49 (84 %) clinically significant mono- and polymicrobial culture results were fully predictive of culture-based identification to bacterial species level at a mean of 1.15 days after specimen collection.CONCLUSIONS: There was a reduction of 1.21 days in the time taken to produce a definitive BCID compared to the previous year, translating into earlier communication of more specific blood culture results to the treating physician. Reduced time to definitive blood culture results has a direct benefit for isolated Australian communities at great distances from specialist hospital services.

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