Field trials of blood culture identification FilmArray in regional Australian hospitals

Nicole M. Bzdyl, Nadezda Urosevic, Ben Payne, Ray Brockenshire, Michael McIntyre, Michael J. Leung, Graham Weaire-Buchanan, Elizabeth Geelhoed, Timothy J J Inglis

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


PURPOSE: In this field trial of rapid blood culture identification (BCID), we aimed to determine whether the improved speed and accuracy of specific BCID predicted in our earlier pilot study could be obtained in regional hospitals by deploying a multiplex PCR FilmArray (Biomerieux, France) capability in their laboratories.

METHODS: We trained local hospital laboratory staff to operate the FilmArray equipment and act on the results. To do this, we integrated the multiplex PCR into the standard laboratory blood culture workflow and reporting procedure.

RESULTS: Of 100 positive blood culture episodes, BCID FilmArray results were correct in all 42 significant monobacterial cultures, with a fully predictive identity in 38 (90.5 %) and a partial identity in another four (9.5 %). There was one major error; a false positive Pseudomonas aeruginosa. The minor errors were the detection of one methicillin-resistant Staphylococcus aureus, which proved to be a methicillin-sensitive S. aureus mixed with a methicillin-resistant coagulase-negative staphylococcus, five false negative coagulase-negative staphylococci and one false negative streptococcus species. We found that 41/49 (84 %) clinically significant mono- and polymicrobial culture results were fully predictive of culture-based identification to bacterial species level at a mean of 1.15 days after specimen collection.

CONCLUSIONS: There was a reduction of 1.21 days in the time taken to produce a definitive BCID compared to the previous year, translating into earlier communication of more specific blood culture results to the treating physician. Reduced time to definitive blood culture results has a direct benefit for isolated Australian communities at great distances from specialist hospital services.

Original languageEnglish
Article number000714
Pages (from-to)669-675
Number of pages7
JournalJournal of Medical Microbiology
Issue number5
Early online date13 Mar 2018
Publication statusPublished - May 2018


Dive into the research topics of 'Field trials of blood culture identification FilmArray in regional Australian hospitals'. Together they form a unique fingerprint.

Cite this