Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumonia in patients with community-acquired pneumonia

C.W.H. Kee; S. Palladino; I. Kay; T.M. Pryce; R. Murray; J. Rello; M. Gallego; M. Lujan; C Munoz-Almagro; Grant Waterer

doi:10.1016/j.diagmicrobio.2007.12.011

Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumonia in patients with community-acquired pneumonia

C.W.H. Kee, S. Palladino, I. Kay, T.M. Pryce, R. Murray, J. Rello, M. Gallego, M. Lujan, C Munoz-Almagro, Grant Waterer

Graduate Research School

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We assessed a real-time quantitative polymerase chain reaction (PCR) assay targeting the lytA and ply gene of Streptococcus pneumoniae. Both assays were applied to whole blood samples from 28 adult patients with community-acquired pneumonia. Our findings suggest thelytA PCR is more sensitive, and the quantitative aspect of the assay shows promise as an aid to clinical judgment.

Original language	English
Pages (from-to)	72-75
Journal	Diagnostic Microbiology and Infectious Disease
Volume	61
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2007.12.011
Publication status	Published - 2008

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Kee, C. W. H., Palladino, S., Kay, I., Pryce, T. M., Murray, R., Rello, J., Gallego, M., Lujan, M., Munoz-Almagro, C., & Waterer, G. (2008). Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumonia in patients with community-acquired pneumonia. Diagnostic Microbiology and Infectious Disease, 61(1), 72-75. https://doi.org/10.1016/j.diagmicrobio.2007.12.011

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abstract = "We assessed a real-time quantitative polymerase chain reaction (PCR) assay targeting the lytA and ply gene of Streptococcus pneumoniae. Both assays were applied to whole blood samples from 28 adult patients with community-acquired pneumonia. Our findings suggest thelytA PCR is more sensitive, and the quantitative aspect of the assay shows promise as an aid to clinical judgment.",

author = "C.W.H. Kee and S. Palladino and I. Kay and T.M. Pryce and R. Murray and J. Rello and M. Gallego and M. Lujan and C Munoz-Almagro and Grant Waterer",

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Kee, CWH, Palladino, S, Kay, I, Pryce, TM, Murray, R, Rello, J, Gallego, M, Lujan, M, Munoz-Almagro, C & Waterer, G 2008, 'Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumonia in patients with community-acquired pneumonia', Diagnostic Microbiology and Infectious Disease, vol. 61, no. 1, pp. 72-75. https://doi.org/10.1016/j.diagmicrobio.2007.12.011

TY - JOUR

T1 - Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumonia in patients with community-acquired pneumonia

AU - Kee, C.W.H.

AU - Palladino, S.

AU - Kay, I.

AU - Pryce, T.M.

AU - Murray, R.

AU - Rello, J.

AU - Gallego, M.

AU - Lujan, M.

AU - Munoz-Almagro, C

AU - Waterer, Grant

PY - 2008

Y1 - 2008

N2 - We assessed a real-time quantitative polymerase chain reaction (PCR) assay targeting the lytA and ply gene of Streptococcus pneumoniae. Both assays were applied to whole blood samples from 28 adult patients with community-acquired pneumonia. Our findings suggest thelytA PCR is more sensitive, and the quantitative aspect of the assay shows promise as an aid to clinical judgment.

AB - We assessed a real-time quantitative polymerase chain reaction (PCR) assay targeting the lytA and ply gene of Streptococcus pneumoniae. Both assays were applied to whole blood samples from 28 adult patients with community-acquired pneumonia. Our findings suggest thelytA PCR is more sensitive, and the quantitative aspect of the assay shows promise as an aid to clinical judgment.

U2 - 10.1016/j.diagmicrobio.2007.12.011

DO - 10.1016/j.diagmicrobio.2007.12.011

M3 - Article

SN - 0732-8893

VL - 61

SP - 72

EP - 75

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 1

ER -

Feasibility of real-time polymerase chain reaction in whole blood to identify Streptococcus pneumonia in patients with community-acquired pneumonia

Abstract

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