TY - JOUR
T1 - Feasibility of parental collected nasal swabs for virus detection in young children with cystic fibrosis
AU - Gangell, C.L.
AU - Shackleton, C.E.
AU - Poreddy, S.
AU - Kappers, J.
AU - Gaydon, J.E.
AU - Sloots, T.P.
AU - Stick, Stephen
AU - Ranganathan, S.C.
AU - Sly, P.D.L.
PY - 2014
Y1 - 2014
N2 - © 2014 European Cystic Fibrosis Society. Background: The detrimental role of viruses has been well described in CF, although the pattern of virus infections has not been investigated in a longitudinal study. The primary aim was to determine the feasibility of fortnightly parent collected swabs in young children with CF. Methods: Children under three years with CF were recruited. Nasal swabs were collected by parents every fortnight and during periods of symptoms over 12. months. Nasal swabs were posted and virus detected using real-time PCR. Results: Only 27% of the patients completed the study to 10. months, although 98% of the swabs returned were adequate for analysis. Mould was observed growing on 23% of the returned swabs. There was no evidence to demonstrate relationships with symptoms and viruses, prolonged symptoms, prolonged shedding or patterns of virus infections. Conclusions: This study highlights the need to further investigate the role of viruses in children with CF using a robust method of frequent collection in children for a longitudinal study, with appropriate storage and shipping techniques to avoid mould growth or other potential contaminants.
AB - © 2014 European Cystic Fibrosis Society. Background: The detrimental role of viruses has been well described in CF, although the pattern of virus infections has not been investigated in a longitudinal study. The primary aim was to determine the feasibility of fortnightly parent collected swabs in young children with CF. Methods: Children under three years with CF were recruited. Nasal swabs were collected by parents every fortnight and during periods of symptoms over 12. months. Nasal swabs were posted and virus detected using real-time PCR. Results: Only 27% of the patients completed the study to 10. months, although 98% of the swabs returned were adequate for analysis. Mould was observed growing on 23% of the returned swabs. There was no evidence to demonstrate relationships with symptoms and viruses, prolonged symptoms, prolonged shedding or patterns of virus infections. Conclusions: This study highlights the need to further investigate the role of viruses in children with CF using a robust method of frequent collection in children for a longitudinal study, with appropriate storage and shipping techniques to avoid mould growth or other potential contaminants.
U2 - 10.1016/j.jcf.2014.02.009
DO - 10.1016/j.jcf.2014.02.009
M3 - Article
C2 - 24637444
VL - 13
SP - 661
EP - 666
JO - Journal of Cystic Fibrosis
JF - Journal of Cystic Fibrosis
SN - 1569-1993
IS - 6
ER -