This study proposes a farmland ecological compensation zoning index (FECZI) and a horizontal farmland ecological fiscal payment model to assess the amount of horizontal fiscal payment in the 48 districts of the Wuhan Agglomeration of China. The results indicate that: 14 districts of Wuhan Agglomeration are classified into farmland ecological payment areas and the remaining 34 districts are compensation ones. Moreover, the largest ecological payment area is Hongshan (109,901.05 x 10(4) Yuan), while the lowest is Tieshan (83.69 x 10(4) Yuan). The largest ecological compensation area is Tianmen (-84,076.11 x 10(4) Yuan), while the least is Hannan (-1340.58 x 10(4) Yuan). The findings can be used in the reform of farmland ecological compensation as well as innovation of fiscal payment mechanism in Wuhan Agglomeration, China.