Fabrication of a silver nanoparticle-coated collagen membrane with anti-bacterial and anti-inflammatory activities for guided bone regeneration

Peilin Chen, Zhigang Wu, Alex Leung, Xiaobo Chen, Euphemie Landao-Bassonga, Junjie Gao, Lianzhi Chen, Monica Zheng, Felix Yao, Hong Yang, Lars Lidgren, Brent Allan, Yinong Liu, Tao Wang, Minghao Zheng

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Alveolar bone loss is a common problem that affects dental implant placement. A barrier between the bone substitute and gingiva that can prevent fibro-tissue ingrowth, bacterial infection and induce bone formation is a key factor in improving the success of alveolar ridge reconstruction. This study aims to develop a bioactive collagen barrier material for guided bone regeneration, that is coupled with anti-bacterial and anti-inflammatory properties. We have evaluated two silver coating methods and found controllable and precise coating achieved by sonication compared with sputtering. The optimized AgNP-coated collagen membrane exhibited excellent anti-bacterial effects against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) with limited cellular toxicity. It also displayed effective anti-inflammatory effects by reducing the expression and release of inflammatory cytokines including IL-6 and TNF-alpha. Additionally, AgNP-coated collagen membranes were able to induce osteogenic differentiation of mesenchymal stem cells that guide bone regeneration. These findings demonstrate the potential application of AgNP- coated collagen membranes to prevent infection after bone graft introduction in alveolar ridge reconstruction.

Original languageEnglish
Article number065014
Number of pages11
JournalBiomedical Materials
Volume13
Issue number6
DOIs
Publication statusPublished - Oct 2018

Cite this

@article{4bad6a5ba90e4aabb0a2072004d23466,
title = "Fabrication of a silver nanoparticle-coated collagen membrane with anti-bacterial and anti-inflammatory activities for guided bone regeneration",
abstract = "Alveolar bone loss is a common problem that affects dental implant placement. A barrier between the bone substitute and gingiva that can prevent fibro-tissue ingrowth, bacterial infection and induce bone formation is a key factor in improving the success of alveolar ridge reconstruction. This study aims to develop a bioactive collagen barrier material for guided bone regeneration, that is coupled with anti-bacterial and anti-inflammatory properties. We have evaluated two silver coating methods and found controllable and precise coating achieved by sonication compared with sputtering. The optimized AgNP-coated collagen membrane exhibited excellent anti-bacterial effects against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) with limited cellular toxicity. It also displayed effective anti-inflammatory effects by reducing the expression and release of inflammatory cytokines including IL-6 and TNF-alpha. Additionally, AgNP-coated collagen membranes were able to induce osteogenic differentiation of mesenchymal stem cells that guide bone regeneration. These findings demonstrate the potential application of AgNP- coated collagen membranes to prevent infection after bone graft introduction in alveolar ridge reconstruction.",
keywords = "collagen, silver nanoparticles, anti-bacterial, anti-inflammation, guide bone regeneration, ALVEOLAR RIDGE AUGMENTATION, DIAMINE FLUORIDE, IN-VITRO, CLINICAL-TRIAL, STEM-CELLS, TISSUE, GRAFTS, PROLIFERATION, CARIES, OSTEOMYELITIS",
author = "Peilin Chen and Zhigang Wu and Alex Leung and Xiaobo Chen and Euphemie Landao-Bassonga and Junjie Gao and Lianzhi Chen and Monica Zheng and Felix Yao and Hong Yang and Lars Lidgren and Brent Allan and Yinong Liu and Tao Wang and Minghao Zheng",
year = "2018",
month = "10",
doi = "10.1088/1748-605X/aae15b",
language = "English",
volume = "13",
journal = "Biomedical Materials (Bristol)",
issn = "1748-6041",
publisher = "IOP Publishing",
number = "6",

}

Fabrication of a silver nanoparticle-coated collagen membrane with anti-bacterial and anti-inflammatory activities for guided bone regeneration. / Chen, Peilin; Wu, Zhigang; Leung, Alex; Chen, Xiaobo; Landao-Bassonga, Euphemie; Gao, Junjie; Chen, Lianzhi; Zheng, Monica; Yao, Felix; Yang, Hong; Lidgren, Lars; Allan, Brent; Liu, Yinong; Wang, Tao; Zheng, Minghao.

In: Biomedical Materials, Vol. 13, No. 6, 065014, 10.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Fabrication of a silver nanoparticle-coated collagen membrane with anti-bacterial and anti-inflammatory activities for guided bone regeneration

AU - Chen, Peilin

AU - Wu, Zhigang

AU - Leung, Alex

AU - Chen, Xiaobo

AU - Landao-Bassonga, Euphemie

AU - Gao, Junjie

AU - Chen, Lianzhi

AU - Zheng, Monica

AU - Yao, Felix

AU - Yang, Hong

AU - Lidgren, Lars

AU - Allan, Brent

AU - Liu, Yinong

AU - Wang, Tao

AU - Zheng, Minghao

PY - 2018/10

Y1 - 2018/10

N2 - Alveolar bone loss is a common problem that affects dental implant placement. A barrier between the bone substitute and gingiva that can prevent fibro-tissue ingrowth, bacterial infection and induce bone formation is a key factor in improving the success of alveolar ridge reconstruction. This study aims to develop a bioactive collagen barrier material for guided bone regeneration, that is coupled with anti-bacterial and anti-inflammatory properties. We have evaluated two silver coating methods and found controllable and precise coating achieved by sonication compared with sputtering. The optimized AgNP-coated collagen membrane exhibited excellent anti-bacterial effects against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) with limited cellular toxicity. It also displayed effective anti-inflammatory effects by reducing the expression and release of inflammatory cytokines including IL-6 and TNF-alpha. Additionally, AgNP-coated collagen membranes were able to induce osteogenic differentiation of mesenchymal stem cells that guide bone regeneration. These findings demonstrate the potential application of AgNP- coated collagen membranes to prevent infection after bone graft introduction in alveolar ridge reconstruction.

AB - Alveolar bone loss is a common problem that affects dental implant placement. A barrier between the bone substitute and gingiva that can prevent fibro-tissue ingrowth, bacterial infection and induce bone formation is a key factor in improving the success of alveolar ridge reconstruction. This study aims to develop a bioactive collagen barrier material for guided bone regeneration, that is coupled with anti-bacterial and anti-inflammatory properties. We have evaluated two silver coating methods and found controllable and precise coating achieved by sonication compared with sputtering. The optimized AgNP-coated collagen membrane exhibited excellent anti-bacterial effects against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) with limited cellular toxicity. It also displayed effective anti-inflammatory effects by reducing the expression and release of inflammatory cytokines including IL-6 and TNF-alpha. Additionally, AgNP-coated collagen membranes were able to induce osteogenic differentiation of mesenchymal stem cells that guide bone regeneration. These findings demonstrate the potential application of AgNP- coated collagen membranes to prevent infection after bone graft introduction in alveolar ridge reconstruction.

KW - collagen

KW - silver nanoparticles

KW - anti-bacterial

KW - anti-inflammation

KW - guide bone regeneration

KW - ALVEOLAR RIDGE AUGMENTATION

KW - DIAMINE FLUORIDE

KW - IN-VITRO

KW - CLINICAL-TRIAL

KW - STEM-CELLS

KW - TISSUE

KW - GRAFTS

KW - PROLIFERATION

KW - CARIES

KW - OSTEOMYELITIS

U2 - 10.1088/1748-605X/aae15b

DO - 10.1088/1748-605X/aae15b

M3 - Article

VL - 13

JO - Biomedical Materials (Bristol)

JF - Biomedical Materials (Bristol)

SN - 1748-6041

IS - 6

M1 - 065014

ER -