Expression QTL analysis of glaucoma endophenotypes in the Norfolk Island isolate provides evidence that immune-related genes are associated with optic disc size

Pik Fang Kho, Rodney A. Lea, Miles C. Benton, David Eccles, Larisa M. Haupt, Alex W. Hewitt, Justin C. Sherwin, David A. Mackey, Lyn R. Griffiths

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Abstract

Primary open-angle glaucoma (POAG) is influenced by both genetic and environmental factors. Despite significant progress in identifying genetic variants associated with POAG, there remains a substantial amount of unexplained heritability. Study design features that may enhance knowledge of the genetic architecture include focusing on multiple quantitative traits related to ocular disorders (i.e. endophenotypes), targeting genetic variants that directly influence gene expression (i.e. cis-eQTLs) and utilising genetically isolated populations to reduce genetic and environmental noise and thus enhance association signals. In this study we performed heritability and blood-based eQTL association analysis of five key POAG endophenotypes in 330 individuals from the Norfolk Island (NI) isolate. Results showed evidence of heritability for all five traits, with H2 estimates ranging from 0.35 for intraocular pressure (IOP) to 0.82 for central corneal thickness (CCT) (P < 0.05). The primary finding was for BTN3A2, whereby both cis-SNP and transcript were significantly associated with disc size within a conditional regression model. Specifically, this model included rs853676 (β = 0.23,P = 0.008) and transcript (β = 0.23, P = 0.03). We also observed a cis-SNP association between optic disc size and LPCAT2 independent of transcript (P = 0.0004). These genes have specific functions in immune system pathways and suggest a role for an inherited immune component of POAG risk. This study also demonstrates an alternate approach to understanding the functional genetic basis of POAG and ocular health more generally.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalJournal of Human Genetics
Volume63
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

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Melanesia
Endophenotypes
Optic Disk
Glaucoma
Genes
Single Nucleotide Polymorphism
Intraocular Pressure
Noise
Immune System
Primary Open Angle Glaucoma
Gene Expression
Health
Population

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Fang Kho, Pik ; Lea, Rodney A. ; Benton, Miles C. ; Eccles, David ; Haupt, Larisa M. ; Hewitt, Alex W. ; Sherwin, Justin C. ; Mackey, David A. ; Griffiths, Lyn R. / Expression QTL analysis of glaucoma endophenotypes in the Norfolk Island isolate provides evidence that immune-related genes are associated with optic disc size. In: Journal of Human Genetics. 2018 ; Vol. 63, No. 1. pp. 83-87.
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Expression QTL analysis of glaucoma endophenotypes in the Norfolk Island isolate provides evidence that immune-related genes are associated with optic disc size. / Fang Kho, Pik; Lea, Rodney A.; Benton, Miles C.; Eccles, David; Haupt, Larisa M.; Hewitt, Alex W.; Sherwin, Justin C.; Mackey, David A.; Griffiths, Lyn R.

In: Journal of Human Genetics, Vol. 63, No. 1, 01.01.2018, p. 83-87.

Research output: Contribution to journalArticle

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AU - Fang Kho, Pik

AU - Lea, Rodney A.

AU - Benton, Miles C.

AU - Eccles, David

AU - Haupt, Larisa M.

AU - Hewitt, Alex W.

AU - Sherwin, Justin C.

AU - Mackey, David A.

AU - Griffiths, Lyn R.

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