Expression profiling of metabolic genes in response to methyl jasmonate reveals regulation of genes of primary and secondary sulfur-related pathways in Arabidopsis thaliana

Ricarda Jost, L. Altschmied, E. Bloem, J. Bogs, J. Gershenzon, U. Hahnel, R. Hansch, T. Hartmann, S. Kopriva, C. Kruse, R.R. Mendel, J. Papenbrock, M. Reichelt, H. Rennenberg, E. Schnug, A. Schmidt, S. Textor, J. Tokuhisa, A. Wachter, M. Wirtz & 2 others T. Rausch, R. Hell

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    Abstract

    The treatment of Arabidopsis thaliana with methyl jasmonate was used to investigate the reaction of 2467 selected genes of primary and secondary metabolism by macroarray hybridization. Hierarchical cluster analysis allowed distinctions to be made between diurnally and methyl jasmonate regulated genes in a time course from 30 min to 24 h. 97 and 64 genes were identified that were up- or down-regulated more than 2-fold by methyl jasmonate, respectively. These genes belong to 18 functional categories of which sulfur-related genes were by far strongest affected. Gene expression and metabolite patterns of sulfur metabolism were analysed in detail, since numerous defense compounds contain oxidized or reduced sulfur. Genes encoding key reactions of sulfate reduction as well as of cysteine, methionine and glutathione synthesis were rapidly up-regulated, but none of the known sulfur-deficiency induced sulfate transporter genes. In addition, increased expression of genes of sulfur-rich defense proteins and of enzymes involved in glucosinolate metabolism was observed. In contrast, profiling of primary and secondary sulfur metabolites revealed only an increase in the indole glucosinolate glucobrassicin upon methyl jasmonate treatment. The observed rapid mRNA changes were thus regulated by a signal independent of the known sulfur deficiency response. These results document for the first time how comprehensively the regulation of sulfur-related genes and plant defense are connected. This interaction is discussed as a new approach to differentiate between supply- and demand-driven regulation of the sulfate assimilation pathway.
    Original languageEnglish
    Pages (from-to)491-508
    JournalPhotosynthesis Research
    Volume86
    Issue number3
    DOIs
    Publication statusPublished - 2005

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    methyl jasmonate
    metabolomics
    Gene Expression Profiling
    Sulfur
    Arabidopsis
    sulfur
    Arabidopsis thaliana
    Genes
    genes
    Sulfates
    Glucosinolates
    sulfates
    glucosinolates
    Metabolites
    Metabolism
    metabolism
    glucobrassicin
    metabolites
    Secondary Metabolism
    Gene Expression

    Cite this

    Jost, Ricarda ; Altschmied, L. ; Bloem, E. ; Bogs, J. ; Gershenzon, J. ; Hahnel, U. ; Hansch, R. ; Hartmann, T. ; Kopriva, S. ; Kruse, C. ; Mendel, R.R. ; Papenbrock, J. ; Reichelt, M. ; Rennenberg, H. ; Schnug, E. ; Schmidt, A. ; Textor, S. ; Tokuhisa, J. ; Wachter, A. ; Wirtz, M. ; Rausch, T. ; Hell, R. / Expression profiling of metabolic genes in response to methyl jasmonate reveals regulation of genes of primary and secondary sulfur-related pathways in Arabidopsis thaliana. In: Photosynthesis Research. 2005 ; Vol. 86, No. 3. pp. 491-508.
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    abstract = "The treatment of Arabidopsis thaliana with methyl jasmonate was used to investigate the reaction of 2467 selected genes of primary and secondary metabolism by macroarray hybridization. Hierarchical cluster analysis allowed distinctions to be made between diurnally and methyl jasmonate regulated genes in a time course from 30 min to 24 h. 97 and 64 genes were identified that were up- or down-regulated more than 2-fold by methyl jasmonate, respectively. These genes belong to 18 functional categories of which sulfur-related genes were by far strongest affected. Gene expression and metabolite patterns of sulfur metabolism were analysed in detail, since numerous defense compounds contain oxidized or reduced sulfur. Genes encoding key reactions of sulfate reduction as well as of cysteine, methionine and glutathione synthesis were rapidly up-regulated, but none of the known sulfur-deficiency induced sulfate transporter genes. In addition, increased expression of genes of sulfur-rich defense proteins and of enzymes involved in glucosinolate metabolism was observed. In contrast, profiling of primary and secondary sulfur metabolites revealed only an increase in the indole glucosinolate glucobrassicin upon methyl jasmonate treatment. The observed rapid mRNA changes were thus regulated by a signal independent of the known sulfur deficiency response. These results document for the first time how comprehensively the regulation of sulfur-related genes and plant defense are connected. This interaction is discussed as a new approach to differentiate between supply- and demand-driven regulation of the sulfate assimilation pathway.",
    author = "Ricarda Jost and L. Altschmied and E. Bloem and J. Bogs and J. Gershenzon and U. Hahnel and R. Hansch and T. Hartmann and S. Kopriva and C. Kruse and R.R. Mendel and J. Papenbrock and M. Reichelt and H. Rennenberg and E. Schnug and A. Schmidt and S. Textor and J. Tokuhisa and A. Wachter and M. Wirtz and T. Rausch and R. Hell",
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    Jost, R, Altschmied, L, Bloem, E, Bogs, J, Gershenzon, J, Hahnel, U, Hansch, R, Hartmann, T, Kopriva, S, Kruse, C, Mendel, RR, Papenbrock, J, Reichelt, M, Rennenberg, H, Schnug, E, Schmidt, A, Textor, S, Tokuhisa, J, Wachter, A, Wirtz, M, Rausch, T & Hell, R 2005, 'Expression profiling of metabolic genes in response to methyl jasmonate reveals regulation of genes of primary and secondary sulfur-related pathways in Arabidopsis thaliana' Photosynthesis Research, vol. 86, no. 3, pp. 491-508. https://doi.org/10.1007/s11120-005-7386-8

    Expression profiling of metabolic genes in response to methyl jasmonate reveals regulation of genes of primary and secondary sulfur-related pathways in Arabidopsis thaliana. / Jost, Ricarda; Altschmied, L.; Bloem, E.; Bogs, J.; Gershenzon, J.; Hahnel, U.; Hansch, R.; Hartmann, T.; Kopriva, S.; Kruse, C.; Mendel, R.R.; Papenbrock, J.; Reichelt, M.; Rennenberg, H.; Schnug, E.; Schmidt, A.; Textor, S.; Tokuhisa, J.; Wachter, A.; Wirtz, M.; Rausch, T.; Hell, R.

    In: Photosynthesis Research, Vol. 86, No. 3, 2005, p. 491-508.

    Research output: Contribution to journalArticle

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    T1 - Expression profiling of metabolic genes in response to methyl jasmonate reveals regulation of genes of primary and secondary sulfur-related pathways in Arabidopsis thaliana

    AU - Jost, Ricarda

    AU - Altschmied, L.

    AU - Bloem, E.

    AU - Bogs, J.

    AU - Gershenzon, J.

    AU - Hahnel, U.

    AU - Hansch, R.

    AU - Hartmann, T.

    AU - Kopriva, S.

    AU - Kruse, C.

    AU - Mendel, R.R.

    AU - Papenbrock, J.

    AU - Reichelt, M.

    AU - Rennenberg, H.

    AU - Schnug, E.

    AU - Schmidt, A.

    AU - Textor, S.

    AU - Tokuhisa, J.

    AU - Wachter, A.

    AU - Wirtz, M.

    AU - Rausch, T.

    AU - Hell, R.

    PY - 2005

    Y1 - 2005

    N2 - The treatment of Arabidopsis thaliana with methyl jasmonate was used to investigate the reaction of 2467 selected genes of primary and secondary metabolism by macroarray hybridization. Hierarchical cluster analysis allowed distinctions to be made between diurnally and methyl jasmonate regulated genes in a time course from 30 min to 24 h. 97 and 64 genes were identified that were up- or down-regulated more than 2-fold by methyl jasmonate, respectively. These genes belong to 18 functional categories of which sulfur-related genes were by far strongest affected. Gene expression and metabolite patterns of sulfur metabolism were analysed in detail, since numerous defense compounds contain oxidized or reduced sulfur. Genes encoding key reactions of sulfate reduction as well as of cysteine, methionine and glutathione synthesis were rapidly up-regulated, but none of the known sulfur-deficiency induced sulfate transporter genes. In addition, increased expression of genes of sulfur-rich defense proteins and of enzymes involved in glucosinolate metabolism was observed. In contrast, profiling of primary and secondary sulfur metabolites revealed only an increase in the indole glucosinolate glucobrassicin upon methyl jasmonate treatment. The observed rapid mRNA changes were thus regulated by a signal independent of the known sulfur deficiency response. These results document for the first time how comprehensively the regulation of sulfur-related genes and plant defense are connected. This interaction is discussed as a new approach to differentiate between supply- and demand-driven regulation of the sulfate assimilation pathway.

    AB - The treatment of Arabidopsis thaliana with methyl jasmonate was used to investigate the reaction of 2467 selected genes of primary and secondary metabolism by macroarray hybridization. Hierarchical cluster analysis allowed distinctions to be made between diurnally and methyl jasmonate regulated genes in a time course from 30 min to 24 h. 97 and 64 genes were identified that were up- or down-regulated more than 2-fold by methyl jasmonate, respectively. These genes belong to 18 functional categories of which sulfur-related genes were by far strongest affected. Gene expression and metabolite patterns of sulfur metabolism were analysed in detail, since numerous defense compounds contain oxidized or reduced sulfur. Genes encoding key reactions of sulfate reduction as well as of cysteine, methionine and glutathione synthesis were rapidly up-regulated, but none of the known sulfur-deficiency induced sulfate transporter genes. In addition, increased expression of genes of sulfur-rich defense proteins and of enzymes involved in glucosinolate metabolism was observed. In contrast, profiling of primary and secondary sulfur metabolites revealed only an increase in the indole glucosinolate glucobrassicin upon methyl jasmonate treatment. The observed rapid mRNA changes were thus regulated by a signal independent of the known sulfur deficiency response. These results document for the first time how comprehensively the regulation of sulfur-related genes and plant defense are connected. This interaction is discussed as a new approach to differentiate between supply- and demand-driven regulation of the sulfate assimilation pathway.

    U2 - 10.1007/s11120-005-7386-8

    DO - 10.1007/s11120-005-7386-8

    M3 - Article

    VL - 86

    SP - 491

    EP - 508

    JO - Photosynthesis Research

    JF - Photosynthesis Research

    SN - 0166-8595

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