Expression of monomorphic and polymorphic N-acetyltransferases in human colon

Kenneth Ilett, D.M. Ingram, D.S. Carpenter, C.H. Teitel, N.P. Lang, F.F. Kadlubar, R.F. Minchin

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Abstract

The metabolism of sulfamethazine (SMZ) and p-aminobenzoic acid (PABA) by N-acetyltransferase (NAT) was measured in human colorectal cytosols from 12 slow and 11 rapid acetylators whose genotype was determined independently by a specific polymerase chain reaction. SMZ metabolism was significantly greater in the rapid than in the slow phenotype (192 +/- 22 versus 94 +/- 11 pmol N-acetylsulfamethazine/min/mg protein), while PABA metabolism was similar in both phenotypes (23.7 +/- 4.4 versus 23.0 +/- 3.9 nmol N-acetyl-p-aminobenzoic acid/min/mg protein). Both monomorphic and polymorphic NAT mRNAs were detected by the polymerase chain reaction in the colorectal mucosa of most samples. The finding that polymorphic NAT is expressed in a phenotype-dependent manner in colorectal mucosa indicates that this tissue has the capacity to participate in local bioactivation of dietary and environmental aryl- or heterocyclic amine carcinogens and may explain, in part, the phenotype-dependent occurrrence of colorectal cancer.
Original languageEnglish
Pages (from-to)914-917
JournalBiochemical Pharmacology
Volume47
DOIs
Publication statusPublished - 1994

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Acetyltransferases
Sulfamethazine
Metabolism
4-Aminobenzoic Acid
Colon
Polymerase chain reaction
Phenotype
Mucous Membrane
Carcinogens
Polymerase Chain Reaction
Amines
Proteins
Cytosol
Tissue
Colorectal Neoplasms
Messenger RNA
Genotype

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Ilett, K., Ingram, D. M., Carpenter, D. S., Teitel, C. H., Lang, N. P., Kadlubar, F. F., & Minchin, R. F. (1994). Expression of monomorphic and polymorphic N-acetyltransferases in human colon. Biochemical Pharmacology, 47, 914-917. https://doi.org/10.1016/0006-2952(94)90493-6
Ilett, Kenneth ; Ingram, D.M. ; Carpenter, D.S. ; Teitel, C.H. ; Lang, N.P. ; Kadlubar, F.F. ; Minchin, R.F. / Expression of monomorphic and polymorphic N-acetyltransferases in human colon. In: Biochemical Pharmacology. 1994 ; Vol. 47. pp. 914-917.
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Ilett, K, Ingram, DM, Carpenter, DS, Teitel, CH, Lang, NP, Kadlubar, FF & Minchin, RF 1994, 'Expression of monomorphic and polymorphic N-acetyltransferases in human colon' Biochemical Pharmacology, vol. 47, pp. 914-917. https://doi.org/10.1016/0006-2952(94)90493-6

Expression of monomorphic and polymorphic N-acetyltransferases in human colon. / Ilett, Kenneth; Ingram, D.M.; Carpenter, D.S.; Teitel, C.H.; Lang, N.P.; Kadlubar, F.F.; Minchin, R.F.

In: Biochemical Pharmacology, Vol. 47, 1994, p. 914-917.

Research output: Contribution to journalArticle

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T1 - Expression of monomorphic and polymorphic N-acetyltransferases in human colon

AU - Ilett, Kenneth

AU - Ingram, D.M.

AU - Carpenter, D.S.

AU - Teitel, C.H.

AU - Lang, N.P.

AU - Kadlubar, F.F.

AU - Minchin, R.F.

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N2 - The metabolism of sulfamethazine (SMZ) and p-aminobenzoic acid (PABA) by N-acetyltransferase (NAT) was measured in human colorectal cytosols from 12 slow and 11 rapid acetylators whose genotype was determined independently by a specific polymerase chain reaction. SMZ metabolism was significantly greater in the rapid than in the slow phenotype (192 +/- 22 versus 94 +/- 11 pmol N-acetylsulfamethazine/min/mg protein), while PABA metabolism was similar in both phenotypes (23.7 +/- 4.4 versus 23.0 +/- 3.9 nmol N-acetyl-p-aminobenzoic acid/min/mg protein). Both monomorphic and polymorphic NAT mRNAs were detected by the polymerase chain reaction in the colorectal mucosa of most samples. The finding that polymorphic NAT is expressed in a phenotype-dependent manner in colorectal mucosa indicates that this tissue has the capacity to participate in local bioactivation of dietary and environmental aryl- or heterocyclic amine carcinogens and may explain, in part, the phenotype-dependent occurrrence of colorectal cancer.

AB - The metabolism of sulfamethazine (SMZ) and p-aminobenzoic acid (PABA) by N-acetyltransferase (NAT) was measured in human colorectal cytosols from 12 slow and 11 rapid acetylators whose genotype was determined independently by a specific polymerase chain reaction. SMZ metabolism was significantly greater in the rapid than in the slow phenotype (192 +/- 22 versus 94 +/- 11 pmol N-acetylsulfamethazine/min/mg protein), while PABA metabolism was similar in both phenotypes (23.7 +/- 4.4 versus 23.0 +/- 3.9 nmol N-acetyl-p-aminobenzoic acid/min/mg protein). Both monomorphic and polymorphic NAT mRNAs were detected by the polymerase chain reaction in the colorectal mucosa of most samples. The finding that polymorphic NAT is expressed in a phenotype-dependent manner in colorectal mucosa indicates that this tissue has the capacity to participate in local bioactivation of dietary and environmental aryl- or heterocyclic amine carcinogens and may explain, in part, the phenotype-dependent occurrrence of colorectal cancer.

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