Expression of monomorphic and polymorphic N-acetyltransferases in human colon

Kenneth Ilett, D.M. Ingram, D.S. Carpenter, C.H. Teitel, N.P. Lang, F.F. Kadlubar, R.F. Minchin

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66 Citations (Scopus)

Abstract

The metabolism of sulfamethazine (SMZ) and p-aminobenzoic acid (PABA) by N-acetyltransferase (NAT) was measured in human colorectal cytosols from 12 slow and 11 rapid acetylators whose genotype was determined independently by a specific polymerase chain reaction. SMZ metabolism was significantly greater in the rapid than in the slow phenotype (192 +/- 22 versus 94 +/- 11 pmol N-acetylsulfamethazine/min/mg protein), while PABA metabolism was similar in both phenotypes (23.7 +/- 4.4 versus 23.0 +/- 3.9 nmol N-acetyl-p-aminobenzoic acid/min/mg protein). Both monomorphic and polymorphic NAT mRNAs were detected by the polymerase chain reaction in the colorectal mucosa of most samples. The finding that polymorphic NAT is expressed in a phenotype-dependent manner in colorectal mucosa indicates that this tissue has the capacity to participate in local bioactivation of dietary and environmental aryl- or heterocyclic amine carcinogens and may explain, in part, the phenotype-dependent occurrrence of colorectal cancer.
Original languageEnglish
Pages (from-to)914-917
JournalBiochemical Pharmacology
Volume47
DOIs
Publication statusPublished - 1994

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