Expression of histone and alkaline phosphatase genes in UMR 106-01 rat osteoblast-like cells exposed to Hoechst dye H33342

Ming Zheng; S. Wysocki; J.M. Papadimitriou; David Wood

doi:10.1002/jcb.240510209

Expression of histone and alkaline phosphatase genes in UMR 106-01 rat osteoblast-like cells exposed to Hoechst dye H33342

Ming Zheng, S. Wysocki, J.M. Papadimitriou, David Wood

Research output: Contribution to journal › Article › peer-review

Abstract

The fluorescent Dye H33342 (H342) is a bis-benzimidazole used for intravital fluorescent staining. In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. The complete loss of histone 2a mRNA occurred after only 20 min of treatment with H342. This effect is unlikely to be a result of inhibition of DNA synthesis, which was only partly suppressed. The mechanism of the action of H342 on histone 2a mRNA is presently unknown.

Original language	English
Pages (from-to)	175-180
Journal	Journal of Cellular Biochemistry
Volume	51
Issue number	2
DOIs	https://doi.org/10.1002/jcb.240510209
Publication status	Published - 1993

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title = "Expression of histone and alkaline phosphatase genes in UMR 106-01 rat osteoblast-like cells exposed to Hoechst dye H33342",

abstract = "The fluorescent Dye H33342 (H342) is a bis-benzimidazole used for intravital fluorescent staining. In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. The complete loss of histone 2a mRNA occurred after only 20 min of treatment with H342. This effect is unlikely to be a result of inhibition of DNA synthesis, which was only partly suppressed. The mechanism of the action of H342 on histone 2a mRNA is presently unknown.",

author = "Ming Zheng and S. Wysocki and J.M. Papadimitriou and David Wood",

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journal = "Journal of Cellular Biochemistry",

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AU - Zheng, Ming

AU - Wysocki, S.

AU - Papadimitriou, J.M.

AU - Wood, David

PY - 1993

Y1 - 1993

N2 - The fluorescent Dye H33342 (H342) is a bis-benzimidazole used for intravital fluorescent staining. In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. The complete loss of histone 2a mRNA occurred after only 20 min of treatment with H342. This effect is unlikely to be a result of inhibition of DNA synthesis, which was only partly suppressed. The mechanism of the action of H342 on histone 2a mRNA is presently unknown.

AB - The fluorescent Dye H33342 (H342) is a bis-benzimidazole used for intravital fluorescent staining. In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. The complete loss of histone 2a mRNA occurred after only 20 min of treatment with H342. This effect is unlikely to be a result of inhibition of DNA synthesis, which was only partly suppressed. The mechanism of the action of H342 on histone 2a mRNA is presently unknown.

U2 - 10.1002/jcb.240510209

DO - 10.1002/jcb.240510209

M3 - Article

VL - 51

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JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

IS - 2

ER -

Expression of histone and alkaline phosphatase genes in UMR 106-01 rat osteoblast-like cells exposed to Hoechst dye H33342

Abstract

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