Expression of histone and alkaline phosphatase genes in UMR 106-01 rat osteoblast-like cells exposed to Hoechst dye H33342

Research output: Contribution to journalArticlepeer-review

Abstract

The fluorescent Dye H33342 (H342) is a bis-benzimidazole used for intravital fluorescent staining. In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. The complete loss of histone 2a mRNA occurred after only 20 min of treatment with H342. This effect is unlikely to be a result of inhibition of DNA synthesis, which was only partly suppressed. The mechanism of the action of H342 on histone 2a mRNA is presently unknown.
Original languageEnglish
Pages (from-to)175-180
JournalJournal of Cellular Biochemistry
Volume51
Issue number2
DOIs
Publication statusPublished - 1993

Fingerprint

Dive into the research topics of 'Expression of histone and alkaline phosphatase genes in UMR 106-01 rat osteoblast-like cells exposed to Hoechst dye H33342'. Together they form a unique fingerprint.

Cite this