Expression of class-3 semaphorins and their receptors in the neonatal and adult rat retina

F. De Winter, Q. Cui, N. Symons, J. Verhaagen, Alan Harvey

    Research output: Contribution to journalArticle

    37 Citations (Scopus)

    Abstract

    PURPOSE. Semaphorins comprise a family of molecules that influence the growth and guidance of neuronal processes. Class-3 semaphorins are secreted proteins, and their effects are mediated by neuropilin (NP) and plexin (Plx) receptors. There is considerable information on mechanisms that influence axonal guidance and plasticity in the mammalian visual system, but a role for semaphorins has received less scrutiny. The purpose of the current study was to survey class-3 semaphorin and cognate receptor expression in young and adult rat retinas.METHOD. The mRNA expression of five class-3 semaphorins (3A, 3B, 3C, 3E, and 3F) and receptor subtypes NP-1 and -2 and plexins A1 and A2 was determined, by using riboprobes and in situ hybridization on cryosections of newborn (postnatal day [P]0), juvenile (P14), and adult rat retinas. Retinal ganglion cells (RGCs) were identified by retrograde labeling after injections of a fluorescent tracer (Fluorogold, FG) into the superior colliculus. Hybridized sections were also immunostained to identify specific retinal cell classes.RESULTS. mRNA expression for all five members of the class-3 semaphorin family was seen in adult FG-labeled RGCs. Qualitatively, expression was highest for semaphorins 3B and 3C, and lowest for 3A. Levels of mRNA expression in RGCs were lower in newborn retinas but were adult-like by P14. Expression by different cell types in the inner nuclear layer was also seen, especially at P14. Expression of NP-2 and PlxA2 mRNAs was evident in developing inner nuclear and ganglion cell layers at birth. Expression increased postnatally and was maintained into adulthood. NP-1 and PlxA1 expression was also present, but at comparatively lower levels.CONCLUSIONS. The presence of class-3 semaphorins and their receptors in neonatal and adult rat retina suggests a potential role for these proteins in retinal development and in the maturation, stabilization, and plasticity of mammalian primary visual pathways.
    Original languageEnglish
    Pages (from-to)4554-4562
    JournalInvestigative ophthalmology & visual science
    Volume45
    Issue number12
    DOIs
    Publication statusPublished - 2004

    Fingerprint

    Semaphorins
    Retina
    Retinal Ganglion Cells
    Neuropilin-2
    Neuropilin-1
    Messenger RNA
    Neuropilins
    Semaphorin-3A
    Newborn Infant
    Visual Pathways
    Superior Colliculi
    varespladib methyl
    Ganglia
    In Situ Hybridization
    Young Adult
    Proteins
    Parturition
    Injections

    Cite this

    De Winter, F. ; Cui, Q. ; Symons, N. ; Verhaagen, J. ; Harvey, Alan. / Expression of class-3 semaphorins and their receptors in the neonatal and adult rat retina. In: Investigative ophthalmology & visual science. 2004 ; Vol. 45, No. 12. pp. 4554-4562.
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    title = "Expression of class-3 semaphorins and their receptors in the neonatal and adult rat retina",
    abstract = "PURPOSE. Semaphorins comprise a family of molecules that influence the growth and guidance of neuronal processes. Class-3 semaphorins are secreted proteins, and their effects are mediated by neuropilin (NP) and plexin (Plx) receptors. There is considerable information on mechanisms that influence axonal guidance and plasticity in the mammalian visual system, but a role for semaphorins has received less scrutiny. The purpose of the current study was to survey class-3 semaphorin and cognate receptor expression in young and adult rat retinas.METHOD. The mRNA expression of five class-3 semaphorins (3A, 3B, 3C, 3E, and 3F) and receptor subtypes NP-1 and -2 and plexins A1 and A2 was determined, by using riboprobes and in situ hybridization on cryosections of newborn (postnatal day [P]0), juvenile (P14), and adult rat retinas. Retinal ganglion cells (RGCs) were identified by retrograde labeling after injections of a fluorescent tracer (Fluorogold, FG) into the superior colliculus. Hybridized sections were also immunostained to identify specific retinal cell classes.RESULTS. mRNA expression for all five members of the class-3 semaphorin family was seen in adult FG-labeled RGCs. Qualitatively, expression was highest for semaphorins 3B and 3C, and lowest for 3A. Levels of mRNA expression in RGCs were lower in newborn retinas but were adult-like by P14. Expression by different cell types in the inner nuclear layer was also seen, especially at P14. Expression of NP-2 and PlxA2 mRNAs was evident in developing inner nuclear and ganglion cell layers at birth. Expression increased postnatally and was maintained into adulthood. NP-1 and PlxA1 expression was also present, but at comparatively lower levels.CONCLUSIONS. The presence of class-3 semaphorins and their receptors in neonatal and adult rat retina suggests a potential role for these proteins in retinal development and in the maturation, stabilization, and plasticity of mammalian primary visual pathways.",
    author = "{De Winter}, F. and Q. Cui and N. Symons and J. Verhaagen and Alan Harvey",
    year = "2004",
    doi = "10.1167/iovs.04-0173",
    language = "English",
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    pages = "4554--4562",
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    Expression of class-3 semaphorins and their receptors in the neonatal and adult rat retina. / De Winter, F.; Cui, Q.; Symons, N.; Verhaagen, J.; Harvey, Alan.

    In: Investigative ophthalmology & visual science, Vol. 45, No. 12, 2004, p. 4554-4562.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Expression of class-3 semaphorins and their receptors in the neonatal and adult rat retina

    AU - De Winter, F.

    AU - Cui, Q.

    AU - Symons, N.

    AU - Verhaagen, J.

    AU - Harvey, Alan

    PY - 2004

    Y1 - 2004

    N2 - PURPOSE. Semaphorins comprise a family of molecules that influence the growth and guidance of neuronal processes. Class-3 semaphorins are secreted proteins, and their effects are mediated by neuropilin (NP) and plexin (Plx) receptors. There is considerable information on mechanisms that influence axonal guidance and plasticity in the mammalian visual system, but a role for semaphorins has received less scrutiny. The purpose of the current study was to survey class-3 semaphorin and cognate receptor expression in young and adult rat retinas.METHOD. The mRNA expression of five class-3 semaphorins (3A, 3B, 3C, 3E, and 3F) and receptor subtypes NP-1 and -2 and plexins A1 and A2 was determined, by using riboprobes and in situ hybridization on cryosections of newborn (postnatal day [P]0), juvenile (P14), and adult rat retinas. Retinal ganglion cells (RGCs) were identified by retrograde labeling after injections of a fluorescent tracer (Fluorogold, FG) into the superior colliculus. Hybridized sections were also immunostained to identify specific retinal cell classes.RESULTS. mRNA expression for all five members of the class-3 semaphorin family was seen in adult FG-labeled RGCs. Qualitatively, expression was highest for semaphorins 3B and 3C, and lowest for 3A. Levels of mRNA expression in RGCs were lower in newborn retinas but were adult-like by P14. Expression by different cell types in the inner nuclear layer was also seen, especially at P14. Expression of NP-2 and PlxA2 mRNAs was evident in developing inner nuclear and ganglion cell layers at birth. Expression increased postnatally and was maintained into adulthood. NP-1 and PlxA1 expression was also present, but at comparatively lower levels.CONCLUSIONS. The presence of class-3 semaphorins and their receptors in neonatal and adult rat retina suggests a potential role for these proteins in retinal development and in the maturation, stabilization, and plasticity of mammalian primary visual pathways.

    AB - PURPOSE. Semaphorins comprise a family of molecules that influence the growth and guidance of neuronal processes. Class-3 semaphorins are secreted proteins, and their effects are mediated by neuropilin (NP) and plexin (Plx) receptors. There is considerable information on mechanisms that influence axonal guidance and plasticity in the mammalian visual system, but a role for semaphorins has received less scrutiny. The purpose of the current study was to survey class-3 semaphorin and cognate receptor expression in young and adult rat retinas.METHOD. The mRNA expression of five class-3 semaphorins (3A, 3B, 3C, 3E, and 3F) and receptor subtypes NP-1 and -2 and plexins A1 and A2 was determined, by using riboprobes and in situ hybridization on cryosections of newborn (postnatal day [P]0), juvenile (P14), and adult rat retinas. Retinal ganglion cells (RGCs) were identified by retrograde labeling after injections of a fluorescent tracer (Fluorogold, FG) into the superior colliculus. Hybridized sections were also immunostained to identify specific retinal cell classes.RESULTS. mRNA expression for all five members of the class-3 semaphorin family was seen in adult FG-labeled RGCs. Qualitatively, expression was highest for semaphorins 3B and 3C, and lowest for 3A. Levels of mRNA expression in RGCs were lower in newborn retinas but were adult-like by P14. Expression by different cell types in the inner nuclear layer was also seen, especially at P14. Expression of NP-2 and PlxA2 mRNAs was evident in developing inner nuclear and ganglion cell layers at birth. Expression increased postnatally and was maintained into adulthood. NP-1 and PlxA1 expression was also present, but at comparatively lower levels.CONCLUSIONS. The presence of class-3 semaphorins and their receptors in neonatal and adult rat retina suggests a potential role for these proteins in retinal development and in the maturation, stabilization, and plasticity of mammalian primary visual pathways.

    U2 - 10.1167/iovs.04-0173

    DO - 10.1167/iovs.04-0173

    M3 - Article

    VL - 45

    SP - 4554

    EP - 4562

    JO - Investigative Ophthalmology & Visual Science (IOVS)

    JF - Investigative Ophthalmology & Visual Science (IOVS)

    SN - 0146-0404

    IS - 12

    ER -