Expression of Catalytically Active Human Cytochrome P450scc in Escherichia coli and Mutagenesis of Isoleucine-462

S.T. Woods, John Sadleir, T. Downs, T. Triantopoulos, M.J. Headlam, Robert Tuckey

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Cytochrome P450scc (P450scc) catalyzes the first step in steroid hormone synthesis, the conversion of cholesterol to pregnenolone, Human P450scc has been poorly studied due to the difficulty of purifying reasonable quantities of enzyme from human tissue. To provide a more convenient source of the human enzyme and to enable structure-function studies to be done using site-directed mutagenesis, we expressed the mature form of human P450scc in Escherichia coli. The expression system enabled us to produce larger quantities of active cytochrome than have previously been isolated from placental mitochondria, The expressed P450scc was purified to near homogeneity and shown to have catalytic properties comparable to the enzyme purified from the human placenta. The mature form of human adrenodoxin was also expressed in E. coli and supported cholesterol side chain cleavage activity with the same V-max as that observed using bovine adrenodoxin but with a higher K-m. Mutation of Ile-462 to Leu in human P450scc caused a decrease in the catalytic rate constant (k(cat)) with cholesterol as substrate, increased the K-m for 22R-hydroxycholesterol, but did not affect the kinetic constants for 20 alpha-hydroxycholesterol. This suggests that Ile-462 lies close to the side chain binding site and that the side chains of cholesterol, 22R-hydroxycholesterol, and 20 alpha-hydroxycholesterol occupy slightly different positions in the active site. (C) 1998 Academic Press.
Original languageEnglish
Pages (from-to)109-115
JournalArchives of Biochemistry and Biophysics
Volume353
Issue number1
DOIs
Publication statusPublished - 1998

Fingerprint Dive into the research topics of 'Expression of Catalytically Active Human Cytochrome P450scc in Escherichia coli and Mutagenesis of Isoleucine-462'. Together they form a unique fingerprint.

Cite this